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Kwon, Hyug Moo
Immunometabolism and Cancer Lab
Research Interests
  • TonEBP, Obesity, Cancer, Chronic inflammatory diseases, Brain disorder, Kidney disorder, Genomic instability

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TonEBP is inhibited by RNA helicase A via interaction involving the E ' F loop

Cited 14 times inthomson ciCited 13 times inthomson ci
Title
TonEBP is inhibited by RNA helicase A via interaction involving the E ' F loop
Author
Colla, ELee, SDSheen, MRWoo, SKKwon, H. Moo
Keywords
E ' F loop; hypertonicity; nuclear factor kappa B (NF-kappa B); RNA helicase A (RHA); small interfering RNA (siRNA); tonicity-responsive-enhancer-binding protein (TonEBP)
Issue Date
2006-01
Publisher
PORTLAND PRESS LTD
Citation
BIOCHEMICAL JOURNAL, v.393, no., pp.411 - 419
Abstract
TonEBP [TonE (tonicity-responsive enhancer)-binding protein] is a transcriptional activator of the Rel family like NF-κ B (nuclear factor κ B) and NFAT (nuclear factor of activated T-cells). TonEBP plays a key role in the protection of cells in the kidney medulla from the deleterious effects of hyperosmolality. This is achieved by enhancing expression of HSP70 (heat-shock protein 70) and other genes whose products drive cellular accumulation of organic osmolytes. TonEBP is stimulated by ambient hypertonicity via multiple pathways that regulate nuclear translocation and transactivation. In the present paper, we report that TonEBP is associated in vivo with RHA (RNA helicase A). The N- and C-termini of RHA bound the E′F loop of the DNA-binding domain of TonEBP. The interaction was not affected by DNA binding or dimerization of TonEBP. Overexpression of RHA inhibited the activity of TonEBP; however, catalytic activity of RHA was dispensable for the inhibition. When the ambient tonicity was raised, the TonEBP-RHA interaction decreased, suggesting that dissociation of RHA is a pathway to stimulate TonEBP. We conclude that the E′F loop of TonEBP interacts with RHA like NFAT and NF-κ B interact with AP1 (activator protein 1) and the high-mobility group protein HMG-I(Y) respectively. While RHA interacts with and stimulates other transcription factors such as CREB (cAMP-response-element-binding protein), NF-κ B and mineralocorticoid receptor, it inhibits TonEBP.
URI
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DOI
10.1042/BJ20051082
ISSN
0264-6021
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BME_Journal Papers
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