Osmolarity in renal medulla of transgenic mice regulates transcription via 5'-flanking region of canine BGT1 gene
Cited 27 times inCited 27 times in
- Osmolarity in renal medulla of transgenic mice regulates transcription via 5'-flanking region of canine BGT1 gene
- Kaneko, T; Takenaka, M; Okabe, M; Yoshimura, Y; Yamauchi, A; Horio, M; Kwon, H. Moo; Handler, JS; Imai, E
- Issue Date
- AMER PHYSIOLOGICAL SOC
- AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY, v.272, no.5, pp.F610 - F616
- Betaine is a major compatible osmolyte accumulated in the mammalian kidney medulla and in Madin-Darby canine kidney cells in response to hypertonicity. The accumulation is the result of an increase in maximal velocity of the Na+- and Cl-coupled betaine transporter designated BGT1. We have previously cloned the canine BGT1 gene and identified a tonicity- responsive enhancer element (TonE) in its 5'-flanking region. Here we report studies of transgenic mice that have in their genome 2.4 kb of the 5'- flanking region of the canine BGT1 gene in front of a chloramphenicol acetyl- transferase (CAT) reporter. Expression of CAT mRNA was detected only in the renal medulla and was increased by experimental manipulations that increase the tonicity of the renal medulla and decreased by manipulations that decrease medullary tonicity. We conclude that the 2.4-kb 5'-flanking region of the BGT1 gene mediates an increase in transcription in response to hyperosmolarity in the renal medulla.
- Appears in Collections:
- BIO_Journal Papers
- Files in This Item:
- There are no files associated with this item.
can give you direct access to the published full text of this article. (UNISTARs only)
Show full item record
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.