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Kim, Jeong Beom
Molecular Biomedicine Lab
Research Interests
  • Patient-specific stem cells, induced pluripotent stem (iPS) cells, direct conversion, 3D bio-printing, Regenerative medicine, Patient-specific drug screening

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Direct monitoring of live human pluripotent stem cells by a highly selective pluripotency sensor

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Title
Direct monitoring of live human pluripotent stem cells by a highly selective pluripotency sensor
Author
Park, Myung RaeNam, DonggyuLee, HyunahChang, Young-TaeZaehres, HolmKim, Jeong Beom
Issue Date
2020-02
Publisher
PERGAMON-ELSEVIER SCIENCE LTD
Citation
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS, v.30, no.16, pp.127347
Abstract
Human pluripotent stem cells (hPSCs) are a useful cell source for regenerative medicine. Despite having a potential of hPSCs for cell-based therapy, there is a need for a selective human pluripotency sensor for monitoring of live hPSCs. Here, we report the discovery of a novel pluripotency sensor (SHI5) from BODIPY-based library by high-throughput cell-based screening and describe the use of SHI5 to identify and isolate human embryonic stem cells and human induced pluripotent stem cells. We demonstrate that SHI5-based assay can be applied to live cells that gain pluripotency in the reprogramming process without any effect on their viability. We also show that SHI5 is internalized through a clathrin-mediated endocytosis pathway. These findings suggest that SHI5 can be an attractive sensor for pluripotency cells during reprogramming. Taken together, SHI5-based screening for hPSCs opens probably unlimited possibilities of detection probe for hPSC therapy via assures their safety issue.
URI
https://scholarworks.unist.ac.kr/handle/201301/47860
URL
https://www.sciencedirect.com/science/article/pii/S0960894X20304583?via%3Dihub
DOI
10.1016/j.bmcl.2020.127347
ISSN
0960-894X
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