There are no files associated with this item.
Full metadata record
DC Field | Value | Language |
---|---|---|
dc.citation.number | 16 | - |
dc.citation.startPage | 127347 | - |
dc.citation.title | BIOORGANIC & MEDICINAL CHEMISTRY LETTERS | - |
dc.citation.volume | 30 | - |
dc.contributor.author | Park, Myung Rae | - |
dc.contributor.author | Nam, Donggyu | - |
dc.contributor.author | Lee, Hyunah | - |
dc.contributor.author | Chang, Young-Tae | - |
dc.contributor.author | Zaehres, Holm | - |
dc.contributor.author | Kim, Jeong Beom | - |
dc.date.accessioned | 2023-12-21T18:06:35Z | - |
dc.date.available | 2023-12-21T18:06:35Z | - |
dc.date.created | 2020-08-10 | - |
dc.date.issued | 2020-02 | - |
dc.description.abstract | Human pluripotent stem cells (hPSCs) are a useful cell source for regenerative medicine. Despite having a potential of hPSCs for cell-based therapy, there is a need for a selective human pluripotency sensor for monitoring of live hPSCs. Here, we report the discovery of a novel pluripotency sensor (SHI5) from BODIPY-based library by high-throughput cell-based screening and describe the use of SHI5 to identify and isolate human embryonic stem cells and human induced pluripotent stem cells. We demonstrate that SHI5-based assay can be applied to live cells that gain pluripotency in the reprogramming process without any effect on their viability. We also show that SHI5 is internalized through a clathrin-mediated endocytosis pathway. These findings suggest that SHI5 can be an attractive sensor for pluripotency cells during reprogramming. Taken together, SHI5-based screening for hPSCs opens probably unlimited possibilities of detection probe for hPSC therapy via assures their safety issue. | - |
dc.identifier.bibliographicCitation | BIOORGANIC & MEDICINAL CHEMISTRY LETTERS, v.30, no.16, pp.127347 | - |
dc.identifier.doi | 10.1016/j.bmcl.2020.127347 | - |
dc.identifier.issn | 0960-894X | - |
dc.identifier.scopusid | 2-s2.0-85086999128 | - |
dc.identifier.uri | https://scholarworks.unist.ac.kr/handle/201301/47860 | - |
dc.identifier.url | https://www.sciencedirect.com/science/article/pii/S0960894X20304583?via%3Dihub | - |
dc.identifier.wosid | 000551073500011 | - |
dc.language | 영어 | - |
dc.publisher | PERGAMON-ELSEVIER SCIENCE LTD | - |
dc.title | Direct monitoring of live human pluripotent stem cells by a highly selective pluripotency sensor | - |
dc.type | Article | - |
dc.description.isOpenAccess | FALSE | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Medicinal; Chemistry, Organic | - |
dc.relation.journalResearchArea | Pharmacology & Pharmacy; Chemistry | - |
dc.type.docType | Article | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.subject.keywordAuthor | Human pluripotent stem cells | - |
dc.subject.keywordAuthor | DOFLA | - |
dc.subject.keywordAuthor | BODIPY | - |
dc.subject.keywordAuthor | Sensor | - |
dc.subject.keywordAuthor | Pluripotency | - |
dc.subject.keywordPlus | DISCOVERY | - |
dc.subject.keywordPlus | LIBRARY | - |
Items in Repository are protected by copyright, with all rights reserved, unless otherwise indicated.
Tel : 052-217-1404 / Email : scholarworks@unist.ac.kr
Copyright (c) 2023 by UNIST LIBRARY. All rights reserved.
ScholarWorks@UNIST was established as an OAK Project for the National Library of Korea.