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Kwon, Hyug Moo
Immunometabolism and Cancer Lab
Research Interests
  • TonEBP, Obesity, Cancer, Chronic inflammatory diseases, Brain disorder, Kidney disorder, Genomic instability

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EXPRESSION OF MADIN-DARBY CANINE KIDNEY-CELL NA+-DEPENDENT AND CL--DEPENDENT TAURINE TRANSPORTER IN XENOPUS-LAEVIS OOCYTES

Cited 44 times inthomson ciCited 22 times inthomson ci
Title
EXPRESSION OF MADIN-DARBY CANINE KIDNEY-CELL NA+-DEPENDENT AND CL--DEPENDENT TAURINE TRANSPORTER IN XENOPUS-LAEVIS OOCYTES
Author
UCHIDA, SKwon, H. MooPRESTON, ASHANDLER, JS
Issue Date
1991-05
Publisher
AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
Citation
JOURNAL OF BIOLOGICAL CHEMISTRY, v.266, no.15, pp.9605 - 9609
Abstract
Expression of a Madin-Darby canine kidney (MDCK) cell taurine transporter was examined in Xenopus oocytes that had been injected with poly(A)+ RNA extracted from MDCK cells. Compared with water-injected oocytes, injection of total poly(A)+ RNA resulted in an increase in Na+-dependent taurine uptake which was directly related to the amount of RNA injected. The magnitude of expression in poly(A)+ RNA-injected oocytes was 5-10-fold higher than that of water-injected oocytes. Since the Vmax of taurine uptake in MDCK cells is increased by culture in hypertonic medium, we compared oocyte taurine uptake after injection with poly(A)+ RNA from MDCK cells cultured in hypertonic medium with uptake in oocytes injected with poly(A)+ RNA from MDCK cells cultured in isotonic medium. Poly(A)+ RNA from hypertonic cells elicited twice the taurine uptake elicited by poly(A)+ RNA from isotonic cells. The transporter expressed in oocytes was like that in MDCK cells: it was completely dependent on external sodium and was also anion dependent (Cl- ≥ Br- > SCN- ≫ gluconate-). Other β-amino acids, β-alanine and hypotaurine, inhibited taurine uptake, but L-alanine and 2-(methylamino) isobutyric acid did not. The apparent Km of the transporter was 7.0 μM. After size fractionation on a sucrose density gradient, poly(A)+ RNA encoding for the MDCK taurine transporter was found in the fraction whose average size was 4.4 kilobases.
URI
https://scholarworks.unist.ac.kr/handle/201301/4740
URL
http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=0025914429
ISSN
0021-9258
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BIO_Journal Papers
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