Dielectrophoresis is one of the most effective technologies for separating a species of particle or cell from a heterogeneous bioparticle mixture in a microfluidic channel. If the frequency of an applied AC voltage is tuned properly, targeted particles can be concentrated in the device while the others thrown away due to the opposite direction of the applied dielectrophoretic forces between them. In this work, we conducted a 3D simulation on a 2D planar microelectrode pattern located on the channel bottom for the better concentration of more targeted bacteria.