Implementation of P22 Viral Capsids As Intravascular Magnetic Resonance T-1 Contrast Conjugates via Site-Selective Attachment of Gd(III)-Chelating Agents
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- Implementation of P22 Viral Capsids As Intravascular Magnetic Resonance T-1 Contrast Conjugates via Site-Selective Attachment of Gd(III)-Chelating Agents
- Min, Junseon; Jung, Hoesu; Shin, Hyun-Hee; Cho, Gyunggoo; Cho, HyungJoon; Kang, Sebyung
- Contrast Enhancement; Exterior surfaces; Ferritin protein; Isotropic resolution; Macromolecular conjugates; Outer diameters; Relaxation rates; Site-selective attachment
- Issue Date
- AMER CHEMICAL SOC
- BIOMACROMOLECULES, v.14, no.7, pp.2332 - 2339
- P22 viral capsids and ferritin protein cages are utilized as templating macromolecules to conjugate Gd(III)-chelating agent complexes, and we systematically investigates the effects of the macromolecules' size and the conjugation positions of Gd(III)-chelating agents on the magnetic resonance (MR) relaxivities and the resulting image contrasts. The relaxivity values of the Gd(III)-chelating agent-conjugated P22 viral capsids (outer diameter: 64 nm) are dramatically increased as compared to both free Gd(III)-chelating agents and Gd(III)-chelating agent-conjugated ferritins (outer diameter: 12 nm), suggesting that the large sized P22 viral capsids exhibit a much slower tumbling rate, which results in a faster T1 relaxation rate. Gd(III)-chelating agents are attached to either the interior or exterior surface of P22 viral capsids and the conjugation positions of Gd(III)-chelating agents, however, do not have a significant effect on the relaxivity values of the macromolecular conjugates. The contrast enhancement of Gd(III)-chelating agent-conjugated P22 viral capsids is confirmed by in vitro phantom imaging at a short repetition times (TR) and the potential usage of Gd(III)-chelating agent-conjugated P22 viral capsids for in vivo MR imaging is validated by visualizing a mouse's intravascular system, including the carotid, mammary arteries, the jugular vein, and the superficial vessels of the head at an isotropic resolution of 250 μm.
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