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- Cho, Yoon-Kyoung
- FRUITS Lab.
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FULLY AUTOMATED PURIFICATION OF CELL-FREE DNA USING LARGE SILICA BEADS ON A LAB-ON-A-DISC SYSTEM
- Author(s)
- Kim, Chi-Ju, Cho, Yoon-Kyoung
- Issued Date
- 2015-10-27
- Citation
- 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015, pp.993 - 995
- Abstract
- We demonstrate rough silica beads integrated on a lab-on-a-disc for a fully automated and rapid (< 30min) purification of Cell-Free DNA (cfDNA). Our cfDNA purification device was attributed to two major techniques integrated onto lab-disc, aminosilane-mediated bonded lab-on-a-disc is able to withstand solvent with chaotropic reagent and preparation of nano-scratched silica beads provides high surface area for DNA binding. Rough and large silica beads on disc can provide highly effective sample preparation for downstream clinical assays.
- Publisher
- Chemical and Biological Microsystems Society
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