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Supra-blot: an accurate and reliable assay for detecting target proteins with a synthetic host molecule-enzyme hybrid

Author(s)
Sung, GihyunLee, Song-YiKang, Myeong-GyunKim, Kyung LockAn, JaeyeonSim, JaehwanKim, SungwanKim, SeungjoonKo, JaewonRhee, Hyun-WooPark, Kyeng MinKim, Kimoon
Issued Date
2020-02
DOI
10.1039/c9cc09699j
URI
https://scholarworks.unist.ac.kr/handle/201301/31315
Fulltext
https://pubs.rsc.org/en/content/articlelanding/2020/CC/C9CC09699J#!divAbstract
Citation
CHEMICAL COMMUNICATIONS, v.56, no.10, pp.1549 - 1552
Abstract
In accordance with the rapid increase in demand for selective and spatial chemical tagging, and accurate detection of proteins of interest, we develop a sensitive protein detection method, termed "Supra-blot" capitalizing on high-affinity host-guest interaction between cucurbit[7]uril (CB[7]) and adamantylammonium (AdA). The method can directly detect chemically tagged proteins without false-positive signals caused by endogenous biomolecules. Not only a single specific protein, but also spatially localized proteins in cells were labeled with AdA, and selectively detected by a host molecule-enzyme hybrid, CB[7]-conjugated horseradish peroxidase (CB[7]-HRP) generating amplified chemiluminescence signals. This study shows the great potential of Supra-blot for accurate and reliable detection of proteins of interest in cells.
Publisher
ROYAL SOC CHEMISTRY
ISSN
1359-7345
Keyword
ENDOGENOUS BIOTINSTREPTAVIDINTAGTECHNOLOGYPEROXIDASEPEPTIDEAZIDESCELLSPAIR

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