There are no files associated with this item.
Full metadata record
DC Field | Value | Language |
---|---|---|
dc.citation.endPage | 1552 | - |
dc.citation.number | 10 | - |
dc.citation.startPage | 1549 | - |
dc.citation.title | CHEMICAL COMMUNICATIONS | - |
dc.citation.volume | 56 | - |
dc.contributor.author | Sung, Gihyun | - |
dc.contributor.author | Lee, Song-Yi | - |
dc.contributor.author | Kang, Myeong-Gyun | - |
dc.contributor.author | Kim, Kyung Lock | - |
dc.contributor.author | An, Jaeyeon | - |
dc.contributor.author | Sim, Jaehwan | - |
dc.contributor.author | Kim, Sungwan | - |
dc.contributor.author | Kim, Seungjoon | - |
dc.contributor.author | Ko, Jaewon | - |
dc.contributor.author | Rhee, Hyun-Woo | - |
dc.contributor.author | Park, Kyeng Min | - |
dc.contributor.author | Kim, Kimoon | - |
dc.date.accessioned | 2023-12-21T18:07:04Z | - |
dc.date.available | 2023-12-21T18:07:04Z | - |
dc.date.created | 2020-03-02 | - |
dc.date.issued | 2020-02 | - |
dc.description.abstract | In accordance with the rapid increase in demand for selective and spatial chemical tagging, and accurate detection of proteins of interest, we develop a sensitive protein detection method, termed "Supra-blot" capitalizing on high-affinity host-guest interaction between cucurbit[7]uril (CB[7]) and adamantylammonium (AdA). The method can directly detect chemically tagged proteins without false-positive signals caused by endogenous biomolecules. Not only a single specific protein, but also spatially localized proteins in cells were labeled with AdA, and selectively detected by a host molecule-enzyme hybrid, CB[7]-conjugated horseradish peroxidase (CB[7]-HRP) generating amplified chemiluminescence signals. This study shows the great potential of Supra-blot for accurate and reliable detection of proteins of interest in cells. | - |
dc.identifier.bibliographicCitation | CHEMICAL COMMUNICATIONS, v.56, no.10, pp.1549 - 1552 | - |
dc.identifier.doi | 10.1039/c9cc09699j | - |
dc.identifier.issn | 1359-7345 | - |
dc.identifier.uri | https://scholarworks.unist.ac.kr/handle/201301/31315 | - |
dc.identifier.url | https://pubs.rsc.org/en/content/articlelanding/2020/CC/C9CC09699J#!divAbstract | - |
dc.identifier.wosid | 000511328200015 | - |
dc.language | 영어 | - |
dc.publisher | ROYAL SOC CHEMISTRY | - |
dc.title | Supra-blot: an accurate and reliable assay for detecting target proteins with a synthetic host molecule-enzyme hybrid | - |
dc.type | Article | - |
dc.description.isOpenAccess | FALSE | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Multidisciplinary | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.type.docType | Article | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.subject.keywordPlus | ENDOGENOUS BIOTIN | - |
dc.subject.keywordPlus | STREPTAVIDIN | - |
dc.subject.keywordPlus | TAG | - |
dc.subject.keywordPlus | TECHNOLOGY | - |
dc.subject.keywordPlus | PEROXIDASE | - |
dc.subject.keywordPlus | PEPTIDE | - |
dc.subject.keywordPlus | AZIDES | - |
dc.subject.keywordPlus | CELLS | - |
dc.subject.keywordPlus | PAIR | - |
Items in Repository are protected by copyright, with all rights reserved, unless otherwise indicated.
Tel : 052-217-1404 / Email : scholarworks@unist.ac.kr
Copyright (c) 2023 by UNIST LIBRARY. All rights reserved.
ScholarWorks@UNIST was established as an OAK Project for the National Library of Korea.