File Download

There are no files associated with this item.

  • Find it @ UNIST can give you direct access to the published full text of this article. (UNISTARs only)

Views & Downloads

Detailed Information

Cited time in webofscience Cited time in scopus
Metadata Downloads

Full metadata record

DC Field Value Language
dc.citation.endPage 1552 -
dc.citation.number 10 -
dc.citation.startPage 1549 -
dc.citation.title CHEMICAL COMMUNICATIONS -
dc.citation.volume 56 -
dc.contributor.author Sung, Gihyun -
dc.contributor.author Lee, Song-Yi -
dc.contributor.author Kang, Myeong-Gyun -
dc.contributor.author Kim, Kyung Lock -
dc.contributor.author An, Jaeyeon -
dc.contributor.author Sim, Jaehwan -
dc.contributor.author Kim, Sungwan -
dc.contributor.author Kim, Seungjoon -
dc.contributor.author Ko, Jaewon -
dc.contributor.author Rhee, Hyun-Woo -
dc.contributor.author Park, Kyeng Min -
dc.contributor.author Kim, Kimoon -
dc.date.accessioned 2023-12-21T18:07:04Z -
dc.date.available 2023-12-21T18:07:04Z -
dc.date.created 2020-03-02 -
dc.date.issued 2020-02 -
dc.description.abstract In accordance with the rapid increase in demand for selective and spatial chemical tagging, and accurate detection of proteins of interest, we develop a sensitive protein detection method, termed "Supra-blot" capitalizing on high-affinity host-guest interaction between cucurbit[7]uril (CB[7]) and adamantylammonium (AdA). The method can directly detect chemically tagged proteins without false-positive signals caused by endogenous biomolecules. Not only a single specific protein, but also spatially localized proteins in cells were labeled with AdA, and selectively detected by a host molecule-enzyme hybrid, CB[7]-conjugated horseradish peroxidase (CB[7]-HRP) generating amplified chemiluminescence signals. This study shows the great potential of Supra-blot for accurate and reliable detection of proteins of interest in cells. -
dc.identifier.bibliographicCitation CHEMICAL COMMUNICATIONS, v.56, no.10, pp.1549 - 1552 -
dc.identifier.doi 10.1039/c9cc09699j -
dc.identifier.issn 1359-7345 -
dc.identifier.uri https://scholarworks.unist.ac.kr/handle/201301/31315 -
dc.identifier.url https://pubs.rsc.org/en/content/articlelanding/2020/CC/C9CC09699J#!divAbstract -
dc.identifier.wosid 000511328200015 -
dc.language 영어 -
dc.publisher ROYAL SOC CHEMISTRY -
dc.title Supra-blot: an accurate and reliable assay for detecting target proteins with a synthetic host molecule-enzyme hybrid -
dc.type Article -
dc.description.isOpenAccess FALSE -
dc.relation.journalWebOfScienceCategory Chemistry, Multidisciplinary -
dc.relation.journalResearchArea Chemistry -
dc.type.docType Article -
dc.description.journalRegisteredClass scie -
dc.description.journalRegisteredClass scopus -
dc.subject.keywordPlus ENDOGENOUS BIOTIN -
dc.subject.keywordPlus STREPTAVIDIN -
dc.subject.keywordPlus TAG -
dc.subject.keywordPlus TECHNOLOGY -
dc.subject.keywordPlus PEROXIDASE -
dc.subject.keywordPlus PEPTIDE -
dc.subject.keywordPlus AZIDES -
dc.subject.keywordPlus CELLS -
dc.subject.keywordPlus PAIR -

qrcode

Items in Repository are protected by copyright, with all rights reserved, unless otherwise indicated.