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GartnerAnton

Gartner, Anton
DNA Damage Response and Genetic Toxicology
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The MRT-1 nuclease is required for DNA crosslink repair and telomerase activity in vivo in Caenorhabditis elegans

Author(s)
Meier, BettinaBarber, Louise J.Liu, YanShtessel, LudmilaBoulton, Simon J.Gartner, AntonAhmed, Shawn
Issued Date
2009-11
DOI
10.1038/emboj.2009.278
URI
https://scholarworks.unist.ac.kr/handle/201301/31002
Fulltext
https://www.embopress.org/doi/10.1038/emboj.2009.278
Citation
EMBO JOURNAL, v.28, no.22, pp.3549 - 3563
Abstract
The telomerase reverse transcriptase adds de novo DNA repeats to chromosome termini. Here we define Caenorhabditis elegans MRT-1 as a novel factor required for telomerase-mediated telomere replication and the DNA-damage response. MRT-1 is composed of an N-terminal domain homologous to the second OB-fold of POT1 telomere-binding proteins and a C-terminal SNM1 family nuclease domain, which confer single-strand DNA-binding and processive 3'-to-5' exonuclease activity, respectively. Furthermore, telomerase activity in vivo depends on a functional MRT-1 OB-fold. We show that MRT-1 acts in the same telomere replication pathway as telomerase and the 9-1-1 DNA-damage response complex. MRT-1 is dispensable for DNA double-strand break repair, but functions with the 9-1-1 complex to promote DNA interstrand cross-link (ICL) repair. Our data reveal MRT-1 as a dual-domain protein required for telomerase function and ICL repair, which raises the possibility that telomeres and ICL lesions may share a common feature that plays a critical role in de novo telomere repeat addition.
Publisher
WILEY
ISSN
0261-4189
Keyword (Author)
C. elegansICLPOT1SNM1telomerase
Keyword
REVERSE-TRANSCRIPTASEFISSION YEASTMETALLO-BETA-LACTAMASEEND-BINDING-PROTEINDAMAGE CHECKPOINT PROTEINSINGLE-STRANDED-DNAGENOME-WIDE SCREENSACCHAROMYCES-CEREVISIAETETRAHYMENA TELOMERASEC-ELEGANS

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