Deacetylated alpha beta-tubulin acts as a positive regulator of Rheb GTPase through increasing its GTP-loading
Cited 3 times inCited 2 times in
- Deacetylated alpha beta-tubulin acts as a positive regulator of Rheb GTPase through increasing its GTP-loading
- Lee, Mi Nam; Koh, Ara; Park, Dohyun; Jang, Jin-Hyeok; Kwak, Dongoh; Jeon, Hyeona; Kim, Jaeyoon; Choi, Eun-Jeong; Jeong, Heeyoon; Suh, Pann-Ghill; Ryu, Sung Ho
- Issue Date
- ELSEVIER SCIENCE INC
- CELLULAR SIGNALLING, v.25, no.2, pp.539 - 551
- Ras homolog enriched in brain (Rheb) regulates diverse cellular functions by modulating its nucleotide-bound status. Although Rheb contains a high basal GTP level, the regulatory mechanism of Rheb is not well understood. In this study, we propose soluble alpha beta-tubulin acts as a constitutively active Rheb activator, which may explain the reason why Rheb has a high basal GTP levels. We found that soluble alpha beta-tubulin is a direct Rheb-binding protein and that its deacetylated form has a high binding affinity for Rheb. Modulation of both soluble and acetylated alpha beta-tubulin levels affects the level of GTP-bound Rheb. This occurs in the mitotic phase in which the level of acetylated alpha beta-tubulin is increased but that of GTP-bound Rheb is decreased. Constitutively active Rheb-overexpressing cells showed an abnormal mitotic progression, suggesting the deacetylated alpha beta-tubulin-mediated regulation of Rheb status may be important for proper mitotic progression. Taken together, we propose that deacetylated soluble alpha beta-tubulin is a novel type of positive regulator of Rheb and may play a role as a temporal regulator for Rheb during the cell cycle.
- Appears in Collections:
- BIO_Journal Papers
- Files in This Item:
- There are no files associated with this item.
can give you direct access to the published full text of this article. (UNISTARs only)
Show full item record
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.