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Myung, Kyungjae
Center for Genomic Integrity
Research Interests
  • DNA Replication, DNA Repair, DNA Recombination, DNA Damage Response, cancer, aging

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CTCF cooperates with CtIP to drive homologous recombination repair of double-strand breaks

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Title
CTCF cooperates with CtIP to drive homologous recombination repair of double-strand breaks
Author
Hwang, Soon YoungKang, Mi AeBaik, Chul JoonLee, YejinHang, Ngo ThanhKim, Byung-GyuHan, Joo SeokJeong, Jae-HoonPark, DaechanMyung, KyungjaeLee, Jong-Soo
Issue Date
2019-09
Publisher
NLM (Medline)
Citation
NUCLEIC ACIDS RESEARCH, v.47, no.17, pp.9160 - 9179
Abstract
The pleiotropic CCCTC-binding factor (CTCF) plays a role in homologous recombination (HR) repair of DNA double-strand breaks (DSBs). However, the precise mechanistic role of CTCF in HR remains largely unclear. Here, we show that CTCF engages in DNA end resection, which is the initial, crucial step in HR, through its interactions with MRE11 and CtIP. Depletion of CTCF profoundly impairs HR and attenuates CtIP recruitment at DSBs. CTCF physically interacts with MRE11 and CtIP and promotes CtIP recruitment to sites of DNA damage. Subsequently, CTCF facilitates DNA end resection to allow HR, in conjunction with MRE11-CtIP. Notably, the zinc finger domain of CTCF binds to both MRE11 and CtIP and enables proficient CtIP recruitment, DNA end resection and HR. The N-terminus of CTCF is able to bind to only MRE11 and its C-terminus is incapable of binding to MRE11 and CtIP, thereby resulting in compromised CtIP recruitment, DSB resection and HR. Overall, this suggests an important function of CTCF in DNA end resection through the recruitment of CtIP at DSBs. Collectively, our findings identify a critical role of CTCF at the first control point in selecting the HR repair pathway.
URI
https://scholarworks.unist.ac.kr/handle/201301/29067
URL
https://academic.oup.com/nar/article/47/17/9160/5538008
DOI
10.1093/nar/gkz639
ISSN
1362-4962
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SLS_Journal Papers
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