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GartnerAnton

Gartner, Anton
DNA Damage Response and Genetic Toxicology
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Caenorhabditis elegans BUB-3 and SAN-1/MAD3 Spindle Assembly Checkpoint Components Are Required for Genome Stability in Response to Treatment with Ionizing Radiation

Author(s)
Bertolini, SimoneWang, BinMeier, BettinaHong, YeGartner, Anton
Issued Date
2017-12
DOI
10.1534/g3.117.1122
URI
https://scholarworks.unist.ac.kr/handle/201301/27440
Fulltext
https://www.g3journal.org/content/7/12/3875
Citation
G3-GENES GENOMES GENETICS, v.7, no.12, pp.3875 - 3885
Abstract
Relatively little is known about the cross-talk between the spindle assembly checkpoint and the DNA damage response, especially in multicellular organisms. We performed a Caenorhabditis elegans forward genetic screen to uncover new genes involved in the repair of DNA damage induced by ionizing radiation. We isolated a mutation, gt2000, which confers hypersensitivity to ionizing radiation and showed that gt2000 introduces a premature stop in bub-3. BUB-3 is a key component of the spindle assembly checkpoint. We provide evidence that BUB-3 acts during development and in the germline; irradiated bub3(gt2000) larvae are developmentally retarded and form abnormal vulvae. Moreover, bub-3(gt2000) embryos sired from irradiated worms show increased levels of lethality. Both bub-3 and san-1 (the C. elegans homolog of MAD3) deletion alleles confer hypersensitivity to ionizing radiation, consistent with the notion that the spindle assembly checkpoint pathway is required for the DNA damage response. bub-3(gt2000) is moderately sensitive to the cross-linking drug cisplatin but not to ultraviolet light or methyl methanesulfonate. This is consistent with a role in dealing with DNA double-strand breaks and not with base damage. Double mutant analysis revealed that bub-3 does not act within any of the three major pathways involved in the repair of double-strand breaks. Finally, the cdc-20 gain-of-function mutant cdc-20/fzy-1(av15), which is refractory to the cell cycle delay conferred by the spindle checkpoint, showed phenotypes similar to bub-3 and san-1 mutants. We speculate that BUB-3 is involved in the DNA damage response through regulation of cell cycle timing.
Publisher
GENETICS SOCIETY AMERICA
ISSN
2160-1836
Keyword (Author)
ionizing radiationspindle assembly checkpointBUB-3SAN-1/MAD-3DNA damage response
Keyword
DNA-DAMAGE RESPONSEINTERSTRAND CROSS-LINKSDOUBLE-STRAND BREAKSCELL-CYCLE ARRESTSACCHAROMYCES-CEREVISIAEBUDDING YEASTC. ELEGANSGERM-LINECHROMOSOMEREPLICATION

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