Flow-enhanced electrochemical immunosensors on centrifugal microfluidic platforms
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- Title
- Flow-enhanced electrochemical immunosensors on centrifugal microfluidic platforms
- Author
- Kim, Tae-Hyeong; Abi-Samra, Kameel; Sunkara, Vijaya; Park, Dong-Kyu; Amasia, Mary; Kim, Nahui; Kim, Jintae; Kim, Hanshin; Madou, Mark; Cho, Yoon-Kyoung
- Issue Date
- 2013-09
- Publisher
- ROYAL SOC CHEMISTRY
- Citation
- LAB ON A CHIP, v.13, no.18, pp.3747 - 3754
- Abstract
- We present a novel fully integrated centrifugal microfluidic device with features for target antigen capture from biological samples, via a bead-based enzyme-linked immune-sorbent assay, and flow-enhanced electrochemical detection. The limit of detection (LOD) of our device for the C-reactive protein (CRP) was determined to be 4.9 pg mL-1, a 17-fold improvement over quantification by optical density. The complete sample-to-answer protocol of our device is fully automated and takes less than 20 min. Overall, the presented microfluidic disc adds to the comparatively small number of fully integrated microfluidic-based platforms that utilize electrochemical detection and exemplifies how electrochemical detection can be enhanced by flow to successfully detect very low levels of biomarkers (e.g. pg mL-1).
- URI
- https://scholarworks.unist.ac.kr/handle/201301/2689
- URL
- http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84882239531
- DOI
- 10.1039/c3lc50374g
- ISSN
- 1473-0197
- Appears in Collections:
- BME_Journal Papers
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