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Hong, Sung You
Synthetic Organic Chemistry Laboratory
Research Interests
  • Synthetic organic chemistry, transition metals, oxidation state

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Fabrication of uniform layer-by-layer assemblies with complementary protein cage nanobuilding blocks via simple His-tag/metal recognition

Cited 3 times inthomson ciCited 1 times inthomson ci
Title
Fabrication of uniform layer-by-layer assemblies with complementary protein cage nanobuilding blocks via simple His-tag/metal recognition
Author
Moon, HyojinKim, Woo GyumLim, SeongdongKang, Young JiShin, Hyun-HeeKo, HyunhyubHong, Sung YouKang, Sebyung
Keywords
QUARTZ-CRYSTAL MICROBALANCE; VIRAL NANOPARTICLES; LUMAZINE SYNTHASE; TAGGED PROTEINS; SURFACES; IMMOBILIZATION; NANOSCALE; ARRAYS; POLYELECTROLYTES; NANOSTRUCTURES
Issue Date
2013-09
Publisher
ROYAL SOC CHEMISTRY
Citation
JOURNAL OF MATERIALS CHEMISTRY B, v.1, no.35, pp.4504 - 4510
Abstract
A capsid-forming enzyme, lumazine synthase isolated from hyperthermophile Aquifex aeolicus (AaLS), is prepared and utilized as a template for constructing nanobuilding blocks to fabricate uniform layer-by-layer (LbL) assemblies. Two functionally complementary AaLS protein cage nanoparticles (PCNs) are generated either by genetically introducing His-tags on the surface of wild-type AaLS PCNs or by chemically attaching metal chelates (Ni–NTA moiety) to the surface of cysteine-bearing AaLS PCNs individually. The multivalent displays of His-tags (AaLS-His6 PCN) and Ni–NTA ligands (AaLS-NTA–Ni PCN) on the surface of each complementary AaLS PCN are successfully demonstrated by mass spectrometric and surface plasmon resonance analyses. By using these two complementary AaLS PCNs, uniform LbL assemblies are constructed via simple recognition between His-tags and metal chelates without the aid of additional binding mediators. This approach illustrates the potential of fabricating uniform nanostructures using protein-based hybrid functional nanobuilding blocks.
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DOI
10.1039/c3tb20554a
ISSN
2050-750X
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SLS_Journal Papers
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