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Cho, Seung Woo
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DNA-free genome editing in plants with preassembled CRISPR-Cas9 ribonucleoproteins

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Title
DNA-free genome editing in plants with preassembled CRISPR-Cas9 ribonucleoproteins
Author
Woo, Je WookKim, JungeunIl Kwon, SoonCorvalan, ClaudiaCho, Seung WooKim, HyeranKim, Sang-GyuKim, Sang-TaeChoe, SunghwaKim, Jin-Soo
Issue Date
2015-11
Publisher
NATURE PUBLISHING GROUP
Citation
NATURE BIOTECHNOLOGY, v.33, no.11, pp.1162 - U156
Abstract
Editing plant genomes without introducing foreign DNA into cells may alleviate regulatory concerns related to genetically modified plants. We transfected preassembled complexes of purified Cas9 protein and guide RNA into plant protoplasts of Arabidopsis thaliana, tobacco, lettuce and rice and achieved targeted mutagenesis in regenerated plants at frequencies of up to 46%. The targeted sites contained germline-transmissible small insertions or deletions that are indistinguishable from naturally occurring genetic variation.
URI
https://scholarworks.unist.ac.kr/handle/201301/25764
URL
https://www.nature.com/articles/nbt.3389
DOI
10.1038/nbt.3389
ISSN
1087-0156
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