File Download

There are no files associated with this item.

  • Find it @ UNIST can give you direct access to the published full text of this article. (UNISTARs only)
Related Researcher

조승우

Cho, Seung Woo
Genome Engineering Lab.
Read More

Views & Downloads

Detailed Information

Cited time in webofscience Cited time in scopus
Metadata Downloads

Full metadata record

DC Field Value Language
dc.citation.endPage U156 -
dc.citation.number 11 -
dc.citation.startPage 1162 -
dc.citation.title NATURE BIOTECHNOLOGY -
dc.citation.volume 33 -
dc.contributor.author Woo, Je Wook -
dc.contributor.author Kim, Jungeun -
dc.contributor.author Il Kwon, Soon -
dc.contributor.author Corvalan, Claudia -
dc.contributor.author Cho, Seung Woo -
dc.contributor.author Kim, Hyeran -
dc.contributor.author Kim, Sang-Gyu -
dc.contributor.author Kim, Sang-Tae -
dc.contributor.author Choe, Sunghwa -
dc.contributor.author Kim, Jin-Soo -
dc.date.accessioned 2023-12-22T00:36:43Z -
dc.date.available 2023-12-22T00:36:43Z -
dc.date.created 2019-01-18 -
dc.date.issued 2015-11 -
dc.description.abstract Editing plant genomes without introducing foreign DNA into cells may alleviate regulatory concerns related to genetically modified plants. We transfected preassembled complexes of purified Cas9 protein and guide RNA into plant protoplasts of Arabidopsis thaliana, tobacco, lettuce and rice and achieved targeted mutagenesis in regenerated plants at frequencies of up to 46%. The targeted sites contained germline-transmissible small insertions or deletions that are indistinguishable from naturally occurring genetic variation. -
dc.identifier.bibliographicCitation NATURE BIOTECHNOLOGY, v.33, no.11, pp.1162 - U156 -
dc.identifier.doi 10.1038/nbt.3389 -
dc.identifier.issn 1087-0156 -
dc.identifier.scopusid 2-s2.0-84947255513 -
dc.identifier.uri https://scholarworks.unist.ac.kr/handle/201301/25764 -
dc.identifier.url https://www.nature.com/articles/nbt.3389 -
dc.identifier.wosid 000364916000023 -
dc.language 영어 -
dc.publisher NATURE PUBLISHING GROUP -
dc.title DNA-free genome editing in plants with preassembled CRISPR-Cas9 ribonucleoproteins -
dc.type Article -
dc.description.isOpenAccess FALSE -
dc.description.journalRegisteredClass scie -
dc.description.journalRegisteredClass scopus -
dc.subject.keywordPlus RNA-GUIDED ENDONUCLEASES -
dc.subject.keywordPlus TRANSIENT GENE-EXPRESSION -
dc.subject.keywordPlus HUMAN-CELLS -
dc.subject.keywordPlus CAS9 -
dc.subject.keywordPlus ARABIDOPSIS -
dc.subject.keywordPlus SYSTEM -
dc.subject.keywordPlus PROTOPLASTS -
dc.subject.keywordPlus NUCLEASES -
dc.subject.keywordPlus KNOCKOUT -

qrcode

Items in Repository are protected by copyright, with all rights reserved, unless otherwise indicated.