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Cho, Seung Woo
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CRISPRi-based genome-scale identification of functional long noncoding RNA loci in human cells

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Title
CRISPRi-based genome-scale identification of functional long noncoding RNA loci in human cells
Author
Liu, S. JohnHorlbeck, Max A.Cho, Seung WooBirk, Harjus S.Malatesta, MartinaHe, DanielAttenello, Frank J.Villalta, Jacqueline E.Cho, Min Y.Chen, YuwenMandegar, Mohammad A.Olvera, Michael P.Gilbert, Luke A.Conklin, Bruce R.Chang, Howard Y.Weissman, Jonathan S.Lim, Daniel A.
Issue Date
2017-01
Publisher
AMER ASSOC ADVANCEMENT SCIENCE
Citation
SCIENCE, v.355, no.6320, pp.aah7111
Abstract
The human genome produces thousands of long noncoding RNAs (lncRNAs)-transcripts >200 nucleotides long that do not encode proteins. Although critical roles in normal biology and disease have been revealed for a subset of lncRNAs, the function of the vast majority remains untested. We developed a CRISPR interference (CRISPRi) platform targeting 16,401 lncRNA loci in seven diverse cell lines, including six transformed cell lines and human induced pluripotent stem cells (iPSCs). Large-scale screening identified 499 lncRNA loci required for robust cellular growth, of which 89% showed growth-modifying function exclusively in one cell type. We further found that lncRNA knockdown can perturb complex transcriptional networks in a cell type-specific manner. These data underscore the functional importance and cell type specificity of many lncRNAs.
URI
https://scholarworks.unist.ac.kr/handle/201301/25762
URL
http://science.sciencemag.org/content/355/6320/eaah7111
DOI
10.1126/science.aah7111
ISSN
0036-8075
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BME_Journal Papers
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