CRISPRi-based genome-scale identification of functional long noncoding RNA loci in human cells
Cited 0 times inCited 0 times in
- CRISPRi-based genome-scale identification of functional long noncoding RNA loci in human cells
- Liu, S. John; Horlbeck, Max A.; Cho, Seung Woo; Birk, Harjus S.; Malatesta, Martina; He, Daniel; Attenello, Frank J.; Villalta, Jacqueline E.; Cho, Min Y.; Chen, Yuwen; Mandegar, Mohammad A.; Olvera, Michael P.; Gilbert, Luke A.; Conklin, Bruce R.; Chang, Howard Y.; Weissman, Jonathan S.; Lim, Daniel A.
- Issue Date
- AMER ASSOC ADVANCEMENT SCIENCE
- SCIENCE, v.355, no.6320, pp.aah7111
- The human genome produces thousands of long noncoding RNAs (lncRNAs)-transcripts >200 nucleotides long that do not encode proteins. Although critical roles in normal biology and disease have been revealed for a subset of lncRNAs, the function of the vast majority remains untested. We developed a CRISPR interference (CRISPRi) platform targeting 16,401 lncRNA loci in seven diverse cell lines, including six transformed cell lines and human induced pluripotent stem cells (iPSCs). Large-scale screening identified 499 lncRNA loci required for robust cellular growth, of which 89% showed growth-modifying function exclusively in one cell type. We further found that lncRNA knockdown can perturb complex transcriptional networks in a cell type-specific manner. These data underscore the functional importance and cell type specificity of many lncRNAs.
- Appears in Collections:
- BME_Journal Papers
- Files in This Item:
- There are no files associated with this item.
can give you direct access to the published full text of this article. (UNISTARs only)
Show full item record
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.