There are no files associated with this item.
Full metadata record
DC Field | Value | Language |
---|---|---|
dc.citation.number | 6320 | - |
dc.citation.startPage | aah7111 | - |
dc.citation.title | SCIENCE | - |
dc.citation.volume | 355 | - |
dc.contributor.author | Liu, S. John | - |
dc.contributor.author | Horlbeck, Max A. | - |
dc.contributor.author | Cho, Seung Woo | - |
dc.contributor.author | Birk, Harjus S. | - |
dc.contributor.author | Malatesta, Martina | - |
dc.contributor.author | He, Daniel | - |
dc.contributor.author | Attenello, Frank J. | - |
dc.contributor.author | Villalta, Jacqueline E. | - |
dc.contributor.author | Cho, Min Y. | - |
dc.contributor.author | Chen, Yuwen | - |
dc.contributor.author | Mandegar, Mohammad A. | - |
dc.contributor.author | Olvera, Michael P. | - |
dc.contributor.author | Gilbert, Luke A. | - |
dc.contributor.author | Conklin, Bruce R. | - |
dc.contributor.author | Chang, Howard Y. | - |
dc.contributor.author | Weissman, Jonathan S. | - |
dc.contributor.author | Lim, Daniel A. | - |
dc.date.accessioned | 2023-12-21T22:42:28Z | - |
dc.date.available | 2023-12-21T22:42:28Z | - |
dc.date.created | 2019-01-18 | - |
dc.date.issued | 2017-01 | - |
dc.description.abstract | The human genome produces thousands of long noncoding RNAs (lncRNAs)-transcripts >200 nucleotides long that do not encode proteins. Although critical roles in normal biology and disease have been revealed for a subset of lncRNAs, the function of the vast majority remains untested. We developed a CRISPR interference (CRISPRi) platform targeting 16,401 lncRNA loci in seven diverse cell lines, including six transformed cell lines and human induced pluripotent stem cells (iPSCs). Large-scale screening identified 499 lncRNA loci required for robust cellular growth, of which 89% showed growth-modifying function exclusively in one cell type. We further found that lncRNA knockdown can perturb complex transcriptional networks in a cell type-specific manner. These data underscore the functional importance and cell type specificity of many lncRNAs. | - |
dc.identifier.bibliographicCitation | SCIENCE, v.355, no.6320, pp.aah7111 | - |
dc.identifier.doi | 10.1126/science.aah7111 | - |
dc.identifier.issn | 0036-8075 | - |
dc.identifier.scopusid | 2-s2.0-85008257169 | - |
dc.identifier.uri | https://scholarworks.unist.ac.kr/handle/201301/25762 | - |
dc.identifier.url | http://science.sciencemag.org/content/355/6320/eaah7111 | - |
dc.identifier.wosid | 000391739900002 | - |
dc.language | 영어 | - |
dc.publisher | AMER ASSOC ADVANCEMENT SCIENCE | - |
dc.title | CRISPRi-based genome-scale identification of functional long noncoding RNA loci in human cells | - |
dc.type | Article | - |
dc.description.isOpenAccess | FALSE | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
Items in Repository are protected by copyright, with all rights reserved, unless otherwise indicated.
Tel : 052-217-1404 / Email : scholarworks@unist.ac.kr
Copyright (c) 2023 by UNIST LIBRARY. All rights reserved.
ScholarWorks@UNIST was established as an OAK Project for the National Library of Korea.