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김홍태

Kim, Hongtae
Cancer/DNA damage Lab.
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Cdk1 Protein-mediated Phosphorylation of Receptor-associated Protein 80 (RAP80) Serine 677 Modulates DNA Damage-induced G(2)/M Checkpoint and Cell Survival

Author(s)
Cho, Hyun JungOh, Yun JungHan, Seung HunChung, Hee JinKim, Chang HeeLee, Nam SooKim, Won-JuChoi, Je-MinKim, Hongtae
Issued Date
2013-02
DOI
10.1074/jbc.M112.401299
URI
https://scholarworks.unist.ac.kr/handle/201301/24893
Fulltext
http://www.jbc.org/content/288/6/3768
Citation
JOURNAL OF BIOLOGICAL CHEMISTRY, v.288, no.6, pp.3768 - 3776
Abstract
Post-translational phosphorylation plays critical roles in the assembly of signaling and repair proteins in the DNA damage response pathway. RAP80, a component of the BRCA1-A complex, is crucial in cell cycle checkpoint activation and DNA damage repair. However, its molecular mechanism is unclear. In this study, we identified Cdk1 as a new RAP80-binding protein and demonstrated that the Cdk1-cyclin B-1 complex phosphorylates RAP80 at Ser-677 using an in vitro kinase assay and a phosphopeptide-specific antibody against phospho-Ser-677 of RAP80. RAP80 Ser-677 phosphorylation occurred in the M phase of the cell cycle when Cdk1 was in an active state. In addition, ionizing radiation (IR) induced RAP80 phosphorylation at Ser-677. Mutation of Ser-677 to alanine sensitized cells to IR and functioned in G(2)/M checkpoint control. These results suggest that post-translational phosphorylation of RAP80 by the Cdk1-cyclin B-1 complex is important for RAP80 functional sensitivity to IR and G(2)/M checkpoint control.
Publisher
AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
ISSN
0021-9258
Keyword
TARGETS BRCA1REPAIRCOMPLEXSITESCYCLECANCERSCCDC98

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