Reusable and storable whole-cell microbial biosensors with a microchemostat platform for in situ on-demand heavy metal detection
Cited 0 times inCited 0 times in
- Reusable and storable whole-cell microbial biosensors with a microchemostat platform for in situ on-demand heavy metal detection
- Bae, Juyeol; Lim, Ji-Won; Kim, Taesung
- Issue Date
- ELSEVIER SCIENCE SA
- SENSORS AND ACTUATORS B-CHEMICAL, v.264, pp.372 - 381
- Target analyte detection using whole-cell microbial biosensors can benefit from a microchemostat platform owing to the reduced reagent consumption, a finely controllable environment, and multi-parallel processing. However, the use of the previous microchemostat still has limitations for practical applications, e.g., its single-use design, long time requirements for cell and device preparation, low portability, slow and weak response signals, and the lack of ready-to-use in situ devices. In the present study, we developed a novel microchemostat platform that resolves these issues and enhances the performance of microbial biosensors via its abilities to actively control the cell population and to form a uniform culture environment using a nanoscale hydrodynamic film (NHF). The combination of the microchemostat platform and the microbial biosensors yielded fast and strong signals on demand in response to heavy metal ions (e.g., Pb2+). In addition, the platform enables the long-term on-chip storage of microbial biosensors for over 1 month, without deterioration of growth and detection ability. Lastly, we demonstrated that microbial biosensors can consecutively measure multiple samples by a regeneration process in which the microchemostat platform continuously subcultures the microbial biosensors until the complete recovery of their sensing capabilities. Thus, the microchemostat offers whole-cell microbial biosensors that are fast, ready-to-use, reusable, and storable. It also shows good potential for user-friendly, portable on-site environmental monitoring, particularly where expensive analytical tools and/or instruments for heavy metal ion detection are unavailable.
- Appears in Collections:
- MEN_Journal Papers
- Files in This Item:
- There are no files associated with this item.
can give you direct access to the published full text of this article. (UNISTARs only)
Show full item record
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.