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DC Field | Value | Language |
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dc.citation.endPage | 381 | - |
dc.citation.startPage | 372 | - |
dc.citation.title | SENSORS AND ACTUATORS B-CHEMICAL | - |
dc.citation.volume | 264 | - |
dc.contributor.author | Bae, Juyeol | - |
dc.contributor.author | Lim, Ji-Won | - |
dc.contributor.author | Kim, Taesung | - |
dc.date.accessioned | 2023-12-21T20:38:32Z | - |
dc.date.available | 2023-12-21T20:38:32Z | - |
dc.date.created | 2018-04-02 | - |
dc.date.issued | 2018-07 | - |
dc.description.abstract | Target analyte detection using whole-cell microbial biosensors can benefit from a microchemostat platform owing to the reduced reagent consumption, a finely controllable environment, and multi-parallel processing. However, the use of the previous microchemostat still has limitations for practical applications, e.g., its single-use design, long time requirements for cell and device preparation, low portability, slow and weak response signals, and the lack of ready-to-use in situ devices. In the present study, we developed a novel microchemostat platform that resolves these issues and enhances the performance of microbial biosensors via its abilities to actively control the cell population and to form a uniform culture environment using a nanoscale hydrodynamic film (NHF). The combination of the microchemostat platform and the microbial biosensors yielded fast and strong signals on demand in response to heavy metal ions (e.g., Pb2+). In addition, the platform enables the long-term on-chip storage of microbial biosensors for over 1 month, without deterioration of growth and detection ability. Lastly, we demonstrated that microbial biosensors can consecutively measure multiple samples by a regeneration process in which the microchemostat platform continuously subcultures the microbial biosensors until the complete recovery of their sensing capabilities. Thus, the microchemostat offers whole-cell microbial biosensors that are fast, ready-to-use, reusable, and storable. It also shows good potential for user-friendly, portable on-site environmental monitoring, particularly where expensive analytical tools and/or instruments for heavy metal ion detection are unavailable. | - |
dc.identifier.bibliographicCitation | SENSORS AND ACTUATORS B-CHEMICAL, v.264, pp.372 - 381 | - |
dc.identifier.doi | 10.1016/j.snb.2018.03.001 | - |
dc.identifier.issn | 0925-4005 | - |
dc.identifier.scopusid | 2-s2.0-85043403205 | - |
dc.identifier.uri | https://scholarworks.unist.ac.kr/handle/201301/23924 | - |
dc.identifier.url | https://www.sciencedirect.com/science/article/pii/S0925400518304799?via%3Dihub | - |
dc.identifier.wosid | 000428032900044 | - |
dc.language | 영어 | - |
dc.publisher | ELSEVIER SCIENCE SA | - |
dc.title | Reusable and storable whole-cell microbial biosensors with a microchemostat platform for in situ on-demand heavy metal detection | - |
dc.type | Article | - |
dc.description.isOpenAccess | FALSE | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Analytical; Electrochemistry; Instruments & Instrumentation | - |
dc.relation.journalResearchArea | Chemistry; Electrochemistry; Instruments & Instrumentation | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.subject.keywordAuthor | Microchemostat | - |
dc.subject.keywordAuthor | Microbial biosensor | - |
dc.subject.keywordAuthor | Multi-function | - |
dc.subject.keywordAuthor | On-site detection | - |
dc.subject.keywordPlus | FLUORESCENCE DETECTION SYSTEM | - |
dc.subject.keywordPlus | ESCHERICHIA-COLI | - |
dc.subject.keywordPlus | BACTERIAL | - |
dc.subject.keywordPlus | CHIP | - |
dc.subject.keywordPlus | MICROFLUIDICS | - |
dc.subject.keywordPlus | TEMPERATURES | - |
dc.subject.keywordPlus | CHEMOSTAT | - |
dc.subject.keywordPlus | BIOFILM | - |
dc.subject.keywordPlus | PHASE | - |
dc.subject.keywordPlus | YEAST | - |
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