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김광수

Kim, Kwang S.
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Spectromicroscopic observation of a live single cell in a biocompatible liquid-enclosing graphene system

Author(s)
Yu, Seong UkLee, HwiwonCho, Woo JongKim, ChulhyunKang, Moon CheolShin, Hyun-JoonKim, NamdongHahn, Sei KwangKim, Kwang S.
Issued Date
2018-01
DOI
10.1039/c7nr05223e
URI
https://scholarworks.unist.ac.kr/handle/201301/23218
Fulltext
http://pubs.rsc.org/en/Content/ArticleLanding/2018/NR/C7NR05223E#!divAbstract
Citation
NANOSCALE, v.10, no.1, pp.150 - 157
Abstract
On-the-spot visualization of biochemical responses of intact live cells is vital for a clear understanding of cell biology. The main obstacles for instant visualization of biochemical responses of living cells arise from the lack of a sophisticated detecting technique which can simultaneously provide chemical analysis tools and the biocompatible wet conditions. Here we introduce scanning transmission X-ray microscopy (STXM) combined with a liquid-enclosing graphene system (LGS), offering biocompatible conditions and improved X-ray absorption spectra to probe the chemical responses of live cells under wet conditions. This set-up enables us to probe a subtle change in absorption spectra depending on the oxidation state of a miniscule amount of oxygen in the functional groups present in each cell and its surroundings containing a minimal amount of liquid water. As an example of in situ biochemical responses of wet cells, chemical responses of a single Colo 205 cell are visualized and analyzed using X-ray absorption near the oxygen K-edge. This spectromicroscopic method using LGS can be applied to diverse biological samples under wet conditions for the analysis of their biochemical responses.
Publisher
ROYAL SOC CHEMISTRY
ISSN
2040-3364
Keyword
TRANSMISSION ELECTRON-MICROSCOPYX-RAY MICROSCOPYHYDROGEN-PEROXIDESPECTROSCOPYDEATHFILMSWATERROS

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