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DC Field | Value | Language |
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dc.citation.endPage | 157 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 150 | - |
dc.citation.title | NANOSCALE | - |
dc.citation.volume | 10 | - |
dc.contributor.author | Yu, Seong Uk | - |
dc.contributor.author | Lee, Hwiwon | - |
dc.contributor.author | Cho, Woo Jong | - |
dc.contributor.author | Kim, Chulhyun | - |
dc.contributor.author | Kang, Moon Cheol | - |
dc.contributor.author | Shin, Hyun-Joon | - |
dc.contributor.author | Kim, Namdong | - |
dc.contributor.author | Hahn, Sei Kwang | - |
dc.contributor.author | Kim, Kwang S. | - |
dc.date.accessioned | 2023-12-21T21:15:23Z | - |
dc.date.available | 2023-12-21T21:15:23Z | - |
dc.date.created | 2018-01-12 | - |
dc.date.issued | 2018-01 | - |
dc.description.abstract | On-the-spot visualization of biochemical responses of intact live cells is vital for a clear understanding of cell biology. The main obstacles for instant visualization of biochemical responses of living cells arise from the lack of a sophisticated detecting technique which can simultaneously provide chemical analysis tools and the biocompatible wet conditions. Here we introduce scanning transmission X-ray microscopy (STXM) combined with a liquid-enclosing graphene system (LGS), offering biocompatible conditions and improved X-ray absorption spectra to probe the chemical responses of live cells under wet conditions. This set-up enables us to probe a subtle change in absorption spectra depending on the oxidation state of a miniscule amount of oxygen in the functional groups present in each cell and its surroundings containing a minimal amount of liquid water. As an example of in situ biochemical responses of wet cells, chemical responses of a single Colo 205 cell are visualized and analyzed using X-ray absorption near the oxygen K-edge. This spectromicroscopic method using LGS can be applied to diverse biological samples under wet conditions for the analysis of their biochemical responses. | - |
dc.identifier.bibliographicCitation | NANOSCALE, v.10, no.1, pp.150 - 157 | - |
dc.identifier.doi | 10.1039/c7nr05223e | - |
dc.identifier.issn | 2040-3364 | - |
dc.identifier.scopusid | 2-s2.0-85039147529 | - |
dc.identifier.uri | https://scholarworks.unist.ac.kr/handle/201301/23218 | - |
dc.identifier.url | http://pubs.rsc.org/en/Content/ArticleLanding/2018/NR/C7NR05223E#!divAbstract | - |
dc.identifier.wosid | 000418621000014 | - |
dc.language | 영어 | - |
dc.publisher | ROYAL SOC CHEMISTRY | - |
dc.title | Spectromicroscopic observation of a live single cell in a biocompatible liquid-enclosing graphene system | - |
dc.type | Article | - |
dc.description.isOpenAccess | FALSE | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Multidisciplinary; Nanoscience & Nanotechnology; Materials Science, Multidisciplinary; Physics, Applied | - |
dc.relation.journalResearchArea | Chemistry; Science & Technology - Other Topics; Materials Science; Physics | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.subject.keywordPlus | TRANSMISSION ELECTRON-MICROSCOPY | - |
dc.subject.keywordPlus | X-RAY MICROSCOPY | - |
dc.subject.keywordPlus | HYDROGEN-PEROXIDE | - |
dc.subject.keywordPlus | SPECTROSCOPY | - |
dc.subject.keywordPlus | DEATH | - |
dc.subject.keywordPlus | FILMS | - |
dc.subject.keywordPlus | WATER | - |
dc.subject.keywordPlus | ROS | - |
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