Effect of culture medium on fermentative and CO-dependent H-2 production activity in Citrobacter amalonaticus Y19
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- Effect of culture medium on fermentative and CO-dependent H-2 production activity in Citrobacter amalonaticus Y19
- Ainala, Satish Kumar; Seol, Eunhee; Kim, Jung Rae; Park, Sunghoon
- Citrobacter amalonaticus; Water-gas shift reaction; CODH; CO-Hyd; CooA
- Issue Date
- PERGAMON-ELSEVIER SCIENCE LTD
- INTERNATIONAL JOURNAL OF HYDROGEN ENERGY, v.41, no.16, pp.6734 - 6742
- Citrobacter amalonaticus Y19 is a unique chemoheterotrophic microorganism capable of carbon monoxide (CO)-dependent hydrogen (H-2) production. To improve its biocatalytic potential, the effects of medium composition on cell growth and H-2 production were investigated. Among several carbon sources tested, maltose resulted in the best CO consumption and H-2 production. Meanwhile, L-cysteine supplementation improved cell growth and H-2 production, suggesting that this amino acid stimulates synthesis of Fe-S clusters. When molybdenum and selenium, which are essential cofactors of formate hydrogen lyase (FHL), were added, formate accumulation was reduced. Optimized medium composition improved the activities of CO-dependent H-2 production (6.3-fold), CODH (22.3-fold) and hydrogenase (2.9-fold). The optimized medium composition also enhanced H-2 production in recombinant C. amalonaticus Y19-cooA, which homologously over expresses CooA, the transcriptional activator of the coo operons. Cells grown under this condition exhibited improved stability in the course of repeated-batch CO-dependent H-2 production under non-growing conditions.
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