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DC Field | Value | Language |
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dc.citation.endPage | 6742 | - |
dc.citation.number | 16 | - |
dc.citation.startPage | 6734 | - |
dc.citation.title | INTERNATIONAL JOURNAL OF HYDROGEN ENERGY | - |
dc.citation.volume | 41 | - |
dc.contributor.author | Ainala, Satish Kumar | - |
dc.contributor.author | Seol, Eunhee | - |
dc.contributor.author | Kim, Jung Rae | - |
dc.contributor.author | Park, Sunghoon | - |
dc.date.accessioned | 2023-12-21T23:42:27Z | - |
dc.date.available | 2023-12-21T23:42:27Z | - |
dc.date.created | 2017-02-19 | - |
dc.date.issued | 2016-05 | - |
dc.description.abstract | Citrobacter amalonaticus Y19 is a unique chemoheterotrophic microorganism capable of carbon monoxide (CO)-dependent hydrogen (H-2) production. To improve its biocatalytic potential, the effects of medium composition on cell growth and H-2 production were investigated. Among several carbon sources tested, maltose resulted in the best CO consumption and H-2 production. Meanwhile, L-cysteine supplementation improved cell growth and H-2 production, suggesting that this amino acid stimulates synthesis of Fe-S clusters. When molybdenum and selenium, which are essential cofactors of formate hydrogen lyase (FHL), were added, formate accumulation was reduced. Optimized medium composition improved the activities of CO-dependent H-2 production (6.3-fold), CODH (22.3-fold) and hydrogenase (2.9-fold). The optimized medium composition also enhanced H-2 production in recombinant C. amalonaticus Y19-cooA, which homologously over expresses CooA, the transcriptional activator of the coo operons. Cells grown under this condition exhibited improved stability in the course of repeated-batch CO-dependent H-2 production under non-growing conditions. | - |
dc.identifier.bibliographicCitation | INTERNATIONAL JOURNAL OF HYDROGEN ENERGY, v.41, no.16, pp.6734 - 6742 | - |
dc.identifier.doi | 10.1016/j.ijhydene.2016.03.088 | - |
dc.identifier.issn | 0360-3199 | - |
dc.identifier.scopusid | 2-s2.0-84962092340 | - |
dc.identifier.uri | https://scholarworks.unist.ac.kr/handle/201301/21656 | - |
dc.identifier.url | http://www.sciencedirect.com/science/article/pii/S0360319915308697 | - |
dc.identifier.wosid | 000375336700011 | - |
dc.language | 영어 | - |
dc.publisher | PERGAMON-ELSEVIER SCIENCE LTD | - |
dc.title | Effect of culture medium on fermentative and CO-dependent H-2 production activity in Citrobacter amalonaticus Y19 | - |
dc.type | Article | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.subject.keywordAuthor | Citrobacter amalonaticus | - |
dc.subject.keywordAuthor | Water-gas shift reaction | - |
dc.subject.keywordAuthor | CODH | - |
dc.subject.keywordAuthor | CO-Hyd | - |
dc.subject.keywordAuthor | CooA | - |
dc.subject.keywordPlus | CARBON-MONOXIDE DEHYDROGENASE | - |
dc.subject.keywordPlus | HYDROGEN-PRODUCTION | - |
dc.subject.keywordPlus | ESCHERICHIA-COLI | - |
dc.subject.keywordPlus | EXPRESSION | - |
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