Dramatic effect of single-base mutation on the conformational dynamics of human telomeric G-quadruplex
Cited 0 times inCited 0 times in
- Dramatic effect of single-base mutation on the conformational dynamics of human telomeric G-quadruplex
- Lee, Ja Yil; Kim, D. S.
- Issue Date
- OXFORD UNIV PRESS
- NUCLEIC ACIDS RESEARCH, v.37, no.11, pp.3625 - 3634
- Guanine-rich DNA sequences can form G-quadruplexes. These four-stranded structures are known to form in several genomic regions and to influence certain biological activities. Sometimes, the instability of G-quadruplexes causes the abnormal biological processes. Mutation is a culprit for the destabilization of G-quadruplexes, but the details of mutated G-quadruplexes are poorly understood. In this article, we investigated the conformational dynamics of single-base mutated human telomeric G-quadruplexes in the presence of K with single-molecule FRET spectroscopy. We observed that the replacement of single guanine by thymine in a G-track induces various folded structures, i.e. structural polymorphism. Moreover, direct observation of their dynamics revealed that a single-base mutation causes fast unfolding of folded states under physiological conditions. Furthermore, we found that the degree of destabilization varies according to mutation positions. When the central guanine of a G-track is replaced, the G-quadruplexes unfold quickly at any K concentrations and temperature. Meanwhile, outer-quartet mutated G-quadruplexes have heterogeneous dynamics at intermediate K concentrations and longstanding folded states at high K concentrations. Several factors such as base-stacking interaction and K coordination are responsible for the different dynamics according to the mutation position
- ; Go to Link
- Appears in Collections:
- SLS_Journal Papers
- Files in This Item:
can give you direct access to the published full text of this article. (UNISTARs only)
Show full item record
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.