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DC Field | Value | Language |
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dc.citation.endPage | 3634 | - |
dc.citation.number | 11 | - |
dc.citation.startPage | 3625 | - |
dc.citation.title | NUCLEIC ACIDS RESEARCH | - |
dc.citation.volume | 37 | - |
dc.contributor.author | Lee, Ja Yil | - |
dc.contributor.author | Kim, Dai-Sik | - |
dc.date.accessioned | 2023-12-22T08:06:06Z | - |
dc.date.available | 2023-12-22T08:06:06Z | - |
dc.date.created | 2016-09-30 | - |
dc.date.issued | 2009-06 | - |
dc.description.abstract | Guanine-rich DNA sequences can form G-quadruplexes. These four-stranded structures are known to form in several genomic regions and to influence certain biological activities. Sometimes, the instability of G-quadruplexes causes the abnormal biological processes. Mutation is a culprit for the destabilization of G-quadruplexes, but the details of mutated G-quadruplexes are poorly understood. In this article, we investigated the conformational dynamics of single-base mutated human telomeric G-quadruplexes in the presence of K with single-molecule FRET spectroscopy. We observed that the replacement of single guanine by thymine in a G-track induces various folded structures, i.e. structural polymorphism. Moreover, direct observation of their dynamics revealed that a single-base mutation causes fast unfolding of folded states under physiological conditions. Furthermore, we found that the degree of destabilization varies according to mutation positions. When the central guanine of a G-track is replaced, the G-quadruplexes unfold quickly at any K concentrations and temperature. Meanwhile, outer-quartet mutated G-quadruplexes have heterogeneous dynamics at intermediate K concentrations and longstanding folded states at high K concentrations. Several factors such as base-stacking interaction and K coordination are responsible for the different dynamics according to the mutation position | - |
dc.identifier.bibliographicCitation | NUCLEIC ACIDS RESEARCH, v.37, no.11, pp.3625 - 3634 | - |
dc.identifier.doi | 10.1093/nar/gkp216 | - |
dc.identifier.issn | 0305-1048 | - |
dc.identifier.scopusid | 2-s2.0-67649838577 | - |
dc.identifier.uri | https://scholarworks.unist.ac.kr/handle/201301/20502 | - |
dc.identifier.url | http://nar.oxfordjournals.org/content/37/11/3625 | - |
dc.identifier.wosid | 000267441800020 | - |
dc.language | 영어 | - |
dc.publisher | OXFORD UNIV PRESS | - |
dc.title | Dramatic effect of single-base mutation on the conformational dynamics of human telomeric G-quadruplex | - |
dc.type | Article | - |
dc.description.isOpenAccess | FALSE | - |
dc.description.journalRegisteredClass | scopus | - |
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