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Lee, Ja Yil
School of Life Sciences
Research Interests
  • Investigated eukaryotic homologous recombination.

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DNA Sequence Alignment by Microhomology Sampling during Homologous Recombination

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Title
DNA Sequence Alignment by Microhomology Sampling during Homologous Recombination
Author
Qi, ZhiRedding, SyLee, Ja YilGibb, BryanKwon, YoungHoNiu, HengyaoGaines, William A.Sung, PatrickGreene, Eric C.
Issue Date
2015-02
Publisher
CELL PRESS
Citation
CELL, v.160, no.5, pp.856 - 869
Abstract
Homologous recombination (HR) mediates the exchange of genetic information between sister or homologous chromatids. During HR, members of the RecA/Rad51 family of recombinases must somehow search through vast quantities of DNA sequence to align and pair single-strand DNA (ssDNA) with a homologous double-strand DNA (dsDNA) template. Here, we use single-molecule imaging to visualize Rad51 as it aligns and pairs homologous DNA sequences in real time. We show that Rad51 uses a length-based recognition mechanism while interrogating dsDNA, enabling robust kinetic selection of 8-nucleotide (nt) tracts of microhomology, which kinetically confines the search to sites with a high probability of being a homologous target. Successful pairing with a ninth nucleotide coincides with an additional reduction in binding free energy, and subsequent strand exchange occurs in precise 3-nt steps, reflecting the base triplet organization of the presynaptic complex. These findings provide crucial new insights into the physical and evolutionary underpinnings of DNA recombination
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DOI
10.1016/j.cell.2015.01.029
ISSN
0092-8674
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SLS_Journal Papers
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