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DC Field | Value | Language |
---|---|---|
dc.citation.endPage | 869 | - |
dc.citation.number | 5 | - |
dc.citation.startPage | 856 | - |
dc.citation.title | CELL | - |
dc.citation.volume | 160 | - |
dc.contributor.author | Qi, Zhi | - |
dc.contributor.author | Redding, Sy | - |
dc.contributor.author | Lee, Ja Yil | - |
dc.contributor.author | Gibb, Bryan | - |
dc.contributor.author | Kwon, YoungHo | - |
dc.contributor.author | Niu, Hengyao | - |
dc.contributor.author | Gaines, William A. | - |
dc.contributor.author | Sung, Patrick | - |
dc.contributor.author | Greene, Eric C. | - |
dc.date.accessioned | 2023-12-22T01:39:11Z | - |
dc.date.available | 2023-12-22T01:39:11Z | - |
dc.date.created | 2016-09-30 | - |
dc.date.issued | 2015-02 | - |
dc.description.abstract | Homologous recombination (HR) mediates the exchange of genetic information between sister or homologous chromatids. During HR, members of the RecA/Rad51 family of recombinases must somehow search through vast quantities of DNA sequence to align and pair single-strand DNA (ssDNA) with a homologous double-strand DNA (dsDNA) template. Here, we use single-molecule imaging to visualize Rad51 as it aligns and pairs homologous DNA sequences in real time. We show that Rad51 uses a length-based recognition mechanism while interrogating dsDNA, enabling robust kinetic selection of 8-nucleotide (nt) tracts of microhomology, which kinetically confines the search to sites with a high probability of being a homologous target. Successful pairing with a ninth nucleotide coincides with an additional reduction in binding free energy, and subsequent strand exchange occurs in precise 3-nt steps, reflecting the base triplet organization of the presynaptic complex. These findings provide crucial new insights into the physical and evolutionary underpinnings of DNA recombination | - |
dc.identifier.bibliographicCitation | CELL, v.160, no.5, pp.856 - 869 | - |
dc.identifier.doi | 10.1016/j.cell.2015.01.029 | - |
dc.identifier.issn | 0092-8674 | - |
dc.identifier.scopusid | 2-s2.0-84923369935 | - |
dc.identifier.uri | https://scholarworks.unist.ac.kr/handle/201301/20495 | - |
dc.identifier.url | http://www.sciencedirect.com/science/article/pii/S0092867415000720 | - |
dc.identifier.wosid | 000351116100009 | - |
dc.language | 영어 | - |
dc.publisher | CELL PRESS | - |
dc.title | DNA Sequence Alignment by Microhomology Sampling during Homologous Recombination | - |
dc.type | Article | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.subject.keywordPlus | STRAND EXCHANGE | - |
dc.subject.keywordPlus | SACCHAROMYCES-CEREVISIAE | - |
dc.subject.keywordPlus | MEIOTIC RECOMBINATION | - |
dc.subject.keywordPlus | RECOGNITION | - |
dc.subject.keywordPlus | MECHANISM | - |
dc.subject.keywordPlus | PROTEINS | - |
dc.subject.keywordPlus | BINDING | - |
dc.subject.keywordPlus | SEARCH | - |
dc.subject.keywordPlus | YEAST | - |
dc.subject.keywordPlus | REPLICATION | - |
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