File Download

There are no files associated with this item.

  • Find it @ UNIST can give you direct access to the published full text of this article. (UNISTARs only)
Related Researcher


Lee, Ja Yil
Biochemistry and Molecular Biophysics Lab.
Read More

Views & Downloads

Detailed Information

Cited time in webofscience Cited time in scopus
Metadata Downloads

Full metadata record

DC Field Value Language
dc.citation.endPage 869 -
dc.citation.number 5 -
dc.citation.startPage 856 -
dc.citation.title CELL -
dc.citation.volume 160 - Qi, Zhi - Redding, Sy - Lee, Ja Yil - Gibb, Bryan - Kwon, YoungHo - Niu, Hengyao - Gaines, William A. - Sung, Patrick - Greene, Eric C. - 2023-12-22T01:39:11Z - 2023-12-22T01:39:11Z - 2016-09-30 - 2015-02 -
dc.description.abstract Homologous recombination (HR) mediates the exchange of genetic information between sister or homologous chromatids. During HR, members of the RecA/Rad51 family of recombinases must somehow search through vast quantities of DNA sequence to align and pair single-strand DNA (ssDNA) with a homologous double-strand DNA (dsDNA) template. Here, we use single-molecule imaging to visualize Rad51 as it aligns and pairs homologous DNA sequences in real time. We show that Rad51 uses a length-based recognition mechanism while interrogating dsDNA, enabling robust kinetic selection of 8-nucleotide (nt) tracts of microhomology, which kinetically confines the search to sites with a high probability of being a homologous target. Successful pairing with a ninth nucleotide coincides with an additional reduction in binding free energy, and subsequent strand exchange occurs in precise 3-nt steps, reflecting the base triplet organization of the presynaptic complex. These findings provide crucial new insights into the physical and evolutionary underpinnings of DNA recombination -
dc.identifier.bibliographicCitation CELL, v.160, no.5, pp.856 - 869 -
dc.identifier.doi 10.1016/j.cell.2015.01.029 -
dc.identifier.issn 0092-8674 -
dc.identifier.scopusid 2-s2.0-84923369935 -
dc.identifier.uri -
dc.identifier.url -
dc.identifier.wosid 000351116100009 -
dc.language 영어 -
dc.publisher CELL PRESS -
dc.title DNA Sequence Alignment by Microhomology Sampling during Homologous Recombination -
dc.type Article -
dc.description.journalRegisteredClass scie -
dc.description.journalRegisteredClass scopus -
dc.subject.keywordPlus STRAND EXCHANGE -
dc.subject.keywordPlus SACCHAROMYCES-CEREVISIAE -
dc.subject.keywordPlus MEIOTIC RECOMBINATION -
dc.subject.keywordPlus RECOGNITION -
dc.subject.keywordPlus MECHANISM -
dc.subject.keywordPlus PROTEINS -
dc.subject.keywordPlus BINDING -
dc.subject.keywordPlus SEARCH -
dc.subject.keywordPlus YEAST -
dc.subject.keywordPlus REPLICATION -


Items in Repository are protected by copyright, with all rights reserved, unless otherwise indicated.