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Chun, Se Young
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The Neuromelanin-related T2* Contrast in Postmortem Human Substantia Nigra with 7T MRI

Author(s)
Lee, Jae-HyeokBaek, Sun-YongSong, YoungKyuLim, SujeongLee, HansolNguyen, Minh PhuongKim, Eun-JooHuh, Gi YeongChun, Se YoungCho, Hyungjoon
Issued Date
2016-09
DOI
10.1038/srep32647
URI
https://scholarworks.unist.ac.kr/handle/201301/20414
Fulltext
http://www.nature.com/articles/srep32647
Citation
SCIENTIFIC REPORTS, v.6, pp.32647
Abstract
High field magnetic resonance imaging (MRI)-based delineation of the substantia nigra (SN) and visualization of its inner cellular organization are promising methods for the evaluation of morphological changes associated with neurodegenerative diseases; however, corresponding MR contrasts must be matched and validated with quantitative histological information. Slices from two postmortem SN samples were imaged with a 7 Tesla (7T) MRI with T1 and T2* imaging protocols and then stained with Perl’s Prussian blue, Kluver-Barrera, tyrosine hydroxylase, and calbindin immunohistochemistry in a serial manner. The association between T2* values and quantitative histology was investigated with a co-registration method that accounts for histology slice preparation. The ventral T2* hypointense layers between the SNr and the crus cerebri extended anteriorly to the posterior part of the crus cerebri, which demonstrates the difficulty with an MRI-based delineation of the SN. We found that the paramagnetic hypointense areas within the dorsolateral SN corresponded to clusters of neuromelanin (NM). These NM-rich zones were distinct from the hypointense ventromedial regions with high iron pigments. Nigral T2* imaging at 7T can reflect the density of NM-containing neurons as the metal-bound NM macromolecules may decrease T2* values and cause hypointense signalling in T2* imaging at 7T.
Publisher
NATURE PUBLISHING GROUP
ISSN
2045-2322
Keyword
PARKINSONS-DISEASELOCUS-CERULEUSSENSITIVE MRIHUMAN BRAINPATHOLOGYHISTOLOGYIMAGESSIGNALTIMES

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