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Kim, Yong Hwan
Enzyme and Protein Engineering Lab.
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Oriented Immobilization of Antibody Fragments on Ni-Decorated Single-Walled Carbon Nanotube Devices

Author(s)
Lo, Young-SeopNam, Dong HyunSo, Hye-MiChang, HyunjuKim, Ju-JinKim, Yong HwanLee, Jeong-O
Issued Date
2009-11
DOI
10.1021/nn900540a
URI
https://scholarworks.unist.ac.kr/handle/201301/20383
Fulltext
http://pubs.acs.org/doi/abs/10.1021/nn900540a
Citation
ACS NANO, v.3, no.11, pp.3649 - 3655
Abstract
We herein demonstrate that Ni-decorated single-walled carbon nanotube field effect transistors (SWNT-FETs) combined with antibody fragments can be used as effective biosensing platforms. Nanoscales Ni particles 20 to 60 nm in diameter were formed on the sidewalls of SWNT-FETs using an electrochemical method. Carcinoembryonic antigen (CEA)-binding single chain variable fragments (scFvs) with a hexahistidine tag [(his)](6)] were synthesized using genetic engineering, and ordered immobilization of anti-CEA ScFvs on Ni nanoparticles was achieved by exploiting the specific interaction between hexahistidine and Ni. Whereas randomly oriented anti(EA scFvs did not impart a noticeable change of conductance upon addition of CEA, a clear increase in conductance was observed using Ni-decorated SWNT-FETs functionalized with engineered scFvs
Publisher
AMER CHEMICAL SOC
ISSN
1936-0851

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