Colour-barcoded magnetic microparticles for multiplexed bioassays

Abstract

Encoded particles have a demonstrated value for multiplexed high-throughput bioassays such as drug discovery and clinical diagnostics(1,2). In diverse samples, the ability to use a large number of distinct identification codes on assay particles is important to increase throughput(3). Proper handling schemes are also needed to readout these codes on free-floating probe microparticles. Here we create vivid, free-floating structural coloured particles with multi-axis rotational control using a colour-tunable magnetic material and a new printing method(4). Our colour-barcoded magnetic microparticles offer a coding capacity easily into the billions with distinct magnetic handling capabilities including active positioning for code readouts and active stirring for improved reaction kinetics in microscale environments(5). A DNA hybridization assay is done using the colour-barcoded magnetic microparticles to demonstrate multiplexing capabilities