Fiber composite slices for multiplexed immunoassays
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- Fiber composite slices for multiplexed immunoassays
- Kim, Jiyun; Bae, Sangwook; Song, Seowoo; Chung, Keumsim; Kwon, Sunghoon
- Issue Date
- AMER INST PHYSICS
- BIOMICROFLUIDICS, v.9, no.4, pp.044109 -
- Fabrication methods for the development of multiplexed immunoassay platforms primarily depend on the individual functionalization of reaction chambers to achieve a heterogeneous reacting substrate composition, which increases the overall manufacturing time and cost. Here, we describe a new type of low-cost fabrication method for a scalable immunoassay platform based on cotton threads. The manufacturing process involves the fabrication of functionalized fibers and the arrangement of these fibers into a bundle; this bundle is then sectioned to make microarray-like particles with a predefined surface architecture. With these sections, composed of heterogeneous thread fragments with different types of antibodies, we demonstrated quantitative and 7-plex immunoassays. We expect that this methodology will prove to be a versatile, low-cost, and highly scalable method for the fabrication of multiplexed bioassay platforms. (C) 2015 AIP Publishing LLC
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