In vitro generation of functional dendritic cells differentiated from CD34 negative cells isolated from human umbilical cord blood
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- In vitro generation of functional dendritic cells differentiated from CD34 negative cells isolated from human umbilical cord blood
- Park, Yo Seph; Shin, Changsik; Hwang, Han Sung; Zenke, Martin; Han, Dong Wook; Kang, Young Sun; Ko, Kisung; Do, Yoonkyung; Ko, Kinarm
- Issue Date
- PORTLAND PRESS LTD
- CELL BIOLOGY INTERNATIONAL, v.39, no.9, pp.1080 - 1086
- Dendritic cells (DCs) are the most potent antigen-presenting cells that play a crucial role in the initiation of an immune response. As DC-based therapeutic applications is increasing, large-scale DC production is required for transplantation. Human umbilical cord blood (UCB) has been shown to contain a rare and precious population of hematopoietic stem cells (HSCs), which can give rise to DCs. The CD34 antigen has been widely used as a cell surface marker to identify HSCs. In this study, we used CD34 antibody to isolate CD34+ and CD34- cells and compared the ability to differentiate into DCs. We used a two-step method combined with the magnetic bead sorting system to isolate CD34+ and CD34- cells from human UCB. Analysis of cellular properties and functionality using a migration assay and T cell proliferation assay revealed no significant differences between CD34+ cells and CD34- cells in their ability to generate DCs.
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