BROWSE

Related Researcher

Author's Photo

Do, Yoonkyung
DC-based Immune System & Immunotherapy (DISNI) Lab
Research Interests
  • Study on various subsets of dendritic cells and their immunological functions
  • Vaccine development by targeting pathogenic antigens to distinct DC subsets via anti-DC-subset-specific-receptor monoclonal antibodies
  • Characterization of roles of DCs in tumor microenvironment and tumor metastasis
  • Studies on role of DCs in neuro-related diseases
  • Study DCs in collaboration with Biotechnology or Engineering field

ITEM VIEW & DOWNLOAD

In vitro generation of functional dendritic cells differentiated from CD34 negative cells isolated from human umbilical cord blood

Cited 0 times inthomson ciCited 0 times inthomson ci
Title
In vitro generation of functional dendritic cells differentiated from CD34 negative cells isolated from human umbilical cord blood
Author
Park, Yo SephShin, ChangsikHwang, Han SungZenke, MartinHan, Dong WookKang, Young SunKo, KisungDo, YoonkyungKo, Kinarm
Issue Date
2015-09
Publisher
PORTLAND PRESS LTD
Citation
CELL BIOLOGY INTERNATIONAL, v.39, no.9, pp.1080 - 1086
Abstract
Dendritic cells (DCs) are the most potent antigen-presenting cells that play a crucial role in the initiation of an immune response. As DC-based therapeutic applications is increasing, large-scale DC production is required for transplantation. Human umbilical cord blood (UCB) has been shown to contain a rare and precious population of hematopoietic stem cells (HSCs), which can give rise to DCs. The CD34 antigen has been widely used as a cell surface marker to identify HSCs. In this study, we used CD34 antibody to isolate CD34+ and CD34- cells and compared the ability to differentiate into DCs. We used a two-step method combined with the magnetic bead sorting system to isolate CD34+ and CD34- cells from human UCB. Analysis of cellular properties and functionality using a migration assay and T cell proliferation assay revealed no significant differences between CD34+ cells and CD34- cells in their ability to generate DCs.
URI
https://scholarworks.unist.ac.kr/handle/201301/16998
URL
http://onlinelibrary.wiley.com/doi/10.1002/cbin.10490/abstract
DOI
10.1002/cbin.10490
ISSN
1065-6995
Appears in Collections:
BME_Journal Papers
Files in This Item:
There are no files associated with this item.

find_unist can give you direct access to the published full text of this article. (UNISTARs only)

Show full item record

qrcode

  • mendeley

    citeulike

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

MENU