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In vitro generation of functional dendritic cells differentiated from CD34 negative cells isolated from human umbilical cord blood

Author(s)
Park, Yo SephShin, ChangsikHwang, Han SungZenke, MartinHan, Dong WookKang, Young SunKo, KisungDo, YoonkyungKo, Kinarm
Issued Date
2015-09
DOI
10.1002/cbin.10490
URI
https://scholarworks.unist.ac.kr/handle/201301/16998
Fulltext
http://onlinelibrary.wiley.com/doi/10.1002/cbin.10490/abstract
Citation
CELL BIOLOGY INTERNATIONAL, v.39, no.9, pp.1080 - 1086
Abstract
Dendritic cells (DCs) are the most potent antigen-presenting cells that play a crucial role in the initiation of an immune response. As DC-based therapeutic applications is increasing, large-scale DC production is required for transplantation. Human umbilical cord blood (UCB) has been shown to contain a rare and precious population of hematopoietic stem cells (HSCs), which can give rise to DCs. The CD34 antigen has been widely used as a cell surface marker to identify HSCs. In this study, we used CD34 antibody to isolate CD34+ and CD34- cells and compared the ability to differentiate into DCs. We used a two-step method combined with the magnetic bead sorting system to isolate CD34+ and CD34- cells from human UCB. Analysis of cellular properties and functionality using a migration assay and T cell proliferation assay revealed no significant differences between CD34+ cells and CD34- cells in their ability to generate DCs.
Publisher
PORTLAND PRESS LTD
ISSN
1065-6995
Keyword (Author)
CD34Dendritic cellsDifferentiationHematopoietic stem cellsHuman umbilical cord blood
Keyword
HEMATOPOIETIC STEM-CELLSTRANSPLANTATION TOLERANCELANGERHANS CELLSIDENTIFICATIONPROGENITORSMIGRATIONANTIGEN

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