BROWSE

Related Researcher

Author's Photo

Suh, Pann-Ghill
BioSignal Network Lab (BSN)
Research Interests
  • Signal transduction, cancer, metabolism, phospholipase C

ITEM VIEW & DOWNLOAD

Phospholipase C-delta 1 is activated by capacitative calcium entry that follows phospholipase C-beta activation upon bradykinin stimulation

Cited 97 times inthomson ciCited 97 times inthomson ci
Title
Phospholipase C-delta 1 is activated by capacitative calcium entry that follows phospholipase C-beta activation upon bradykinin stimulation
Author
Kim, Yong-HyunPark, Tae-JuLee, Young HanBaek, Kwang JinSuh, Pann-GhillRyu, Sung HoKim, Kyong-Tai
Issue Date
1999-09
Publisher
AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
Citation
JOURNAL OF BIOLOGICAL CHEMISTRY, v.274, no.37, pp.26127 - 26134
Abstract
To characterize the regulatory mechanism of phospholipase C-delta 1 (PLC-delta 1) in the bradykinin (BK) receptor-mediated signaling pathway, we used a clone of PC12 cells, which stably overexpress PLC-delta 1 (PC12-D1). Stimulation with BK induced a significantly higher Ca2+ elevation and inositol 1,4,5-trisphosphate (IP3) production with a much lower half-maximal effective concentration (EC50) of BK in PC12-D1 cells than in wild type (PC12-W) or vector-transfected (PC12-V) cells. However, BK-induced intracellular Ca2+ release and IF, generation was similar between PC12-V and PC12-D1 cells in the absence of extracellular Ca2+, suggesting that the availability of extracellular Ca2+ is essential to the activation of PLC-delta 1. When PC12-D1 cells were treated with agents that induce Ca2+ influx, more IP3 was produced, suggesting that the Ca2+ entry induces IP3 production in PC12-D1 cells. Furthermore, the additional IP3 production after BK-induced capacitative calcium entry was detected in PC12-D1 cells, suggesting that PLC-delta 1 is mainly activated by capacitative calcium entry. When cells were stimulated with BK in the presence of extracellular Ca2+, [H-3]norepinephrine secretion was much greater from PC12-D1 cells than from PC12-V cells. Our results suggest that PLC-delta 1 is activated by capacitative calcium entry following the activation of PLC-P, additively inducing IF, production and Ca2+ rise in BK-stimulated PC12 cells
URI
https://scholarworks.unist.ac.kr/handle/201301/16485
URL
http://www.jbc.org/content/274/37/26127.long
DOI
10.1074/jbc.274.37.26127
ISSN
0021-9258
Appears in Collections:
BIO_Journal Papers
Files in This Item:
There are no files associated with this item.

find_unist can give you direct access to the published full text of this article. (UNISTARs only)

Show full item record

qrcode

  • mendeley

    citeulike

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

MENU