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DC Field | Value | Language |
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dc.citation.endPage | 26134 | - |
dc.citation.number | 37 | - |
dc.citation.startPage | 26127 | - |
dc.citation.title | JOURNAL OF BIOLOGICAL CHEMISTRY | - |
dc.citation.volume | 274 | - |
dc.contributor.author | Kim, Yong-Hyun | - |
dc.contributor.author | Park, Tae-Ju | - |
dc.contributor.author | Lee, Young Han | - |
dc.contributor.author | Baek, Kwang Jin | - |
dc.contributor.author | Suh, Pann-Ghill | - |
dc.contributor.author | Ryu, Sung Ho | - |
dc.contributor.author | Kim, Kyong-Tai | - |
dc.date.accessioned | 2023-12-22T12:10:20Z | - |
dc.date.available | 2023-12-22T12:10:20Z | - |
dc.date.created | 2015-08-19 | - |
dc.date.issued | 1999-09 | - |
dc.description.abstract | To characterize the regulatory mechanism of phospholipase C-delta 1 (PLC-delta 1) in the bradykinin (BK) receptor-mediated signaling pathway, we used a clone of PC12 cells, which stably overexpress PLC-delta 1 (PC12-D1). Stimulation with BK induced a significantly higher Ca2+ elevation and inositol 1,4,5-trisphosphate (IP3) production with a much lower half-maximal effective concentration (EC50) of BK in PC12-D1 cells than in wild type (PC12-W) or vector-transfected (PC12-V) cells. However, BK-induced intracellular Ca2+ release and IF, generation was similar between PC12-V and PC12-D1 cells in the absence of extracellular Ca2+, suggesting that the availability of extracellular Ca2+ is essential to the activation of PLC-delta 1. When PC12-D1 cells were treated with agents that induce Ca2+ influx, more IP3 was produced, suggesting that the Ca2+ entry induces IP3 production in PC12-D1 cells. Furthermore, the additional IP3 production after BK-induced capacitative calcium entry was detected in PC12-D1 cells, suggesting that PLC-delta 1 is mainly activated by capacitative calcium entry. When cells were stimulated with BK in the presence of extracellular Ca2+, [H-3]norepinephrine secretion was much greater from PC12-D1 cells than from PC12-V cells. Our results suggest that PLC-delta 1 is activated by capacitative calcium entry following the activation of PLC-P, additively inducing IF, production and Ca2+ rise in BK-stimulated PC12 cells | - |
dc.identifier.bibliographicCitation | JOURNAL OF BIOLOGICAL CHEMISTRY, v.274, no.37, pp.26127 - 26134 | - |
dc.identifier.doi | 10.1074/jbc.274.37.26127 | - |
dc.identifier.issn | 0021-9258 | - |
dc.identifier.scopusid | 2-s2.0-0033543237 | - |
dc.identifier.uri | https://scholarworks.unist.ac.kr/handle/201301/16485 | - |
dc.identifier.url | http://www.jbc.org/content/274/37/26127.long | - |
dc.identifier.wosid | 000082469700029 | - |
dc.language | 영어 | - |
dc.publisher | AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC | - |
dc.title | Phospholipase C-delta 1 is activated by capacitative calcium entry that follows phospholipase C-beta activation upon bradykinin stimulation | - |
dc.type | Article | - |
dc.description.journalRegisteredClass | scopus | - |
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