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Bhak, Jong
KOrean GenomIcs Center(KOGIC)
Research Interests
  • Geromics, genomics, bioinformatics, protein Engineering, OMICS

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Kinetics of Zn2+-induced Brain Type Creatine Kinase Unfolding and Aggregation

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Title
Kinetics of Zn2+-induced Brain Type Creatine Kinase Unfolding and Aggregation
Other Titles
Kinetics of Zn2+ -induced Brain Type Creatine Kinase Unfolding and Aggregation.
Author
Mu, HangLue, Zhi-RongPark, DaeuiKim, Byoung-ChulBhak, Jong HwaZou, FeiYang, Jun-MoLi, SenPark, Yong-DooZou, He-ChangZhou, Hai-Meng
Keywords
Creatine kinase;  Brain-type;  Zn2+;  Folding;  Aggregation
Issue Date
2010-03
Publisher
HUMANA PRESS INC
Citation
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, v.160, no.5, pp.1309 - 1320
Abstract
We studied the effect of Zn2+ on the folding and aggregation of brain creatine kinase (CK-BB). We developed a method to purify CK-BB from rabbit brain and conducted inhibition kinetics and unfolding studies of CK-BB. Zn2+ conspicuously aggregated and osmolytes, such as glycine and proline, were able to suppress the formation of aggregates and protect the enzymatic activity against Zn2+. These results suggest that Zn2+ might act as a risk factor for CK-BB in the brain under certain conditions, and some osmolytes may help CK-BB to sustain the active state when Zn2+ is present. Our study provides useful information regarding the effect of Zn2+ on brain-derived metabolic enzymes, especially those that are putatively related to brain disease. Furthermore, our study suggests that although Zn2+ may induce CK-BB inactivation and misfolding, the ability of some abundant proteins and osmolytes to chelate Zn2+ nonspecifically may protect CK-BB and allow it to exist in the active form
URI
https://scholarworks.unist.ac.kr/handle/201301/13221
DOI
10.1007/s12010-009-8574-3
ISSN
0273-2289
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BME_Journal Papers
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