BROWSE

Related Researcher

Author's Photo

Bhak, Jong
KOrean GenomIcs Center(KOGIC)
Research Interests
  • Geromics, genomics, bioinformatics, protein Engineering, OMICS

ITEM VIEW & DOWNLOAD

Prediction and evaluation of protein-protein interaction in keratinocyte differentiation

Cited 9 times inthomson ciCited 9 times inthomson ci
Title
Prediction and evaluation of protein-protein interaction in keratinocyte differentiation
Other Titles
Prediction and evaluation of protein-protein interaction in keratinocyte differentiation.
Author
Yoon, Hyun KyungSohn, Kyung-CheolLee, Jung-SukKim, Yu JinBhak, Jong HwaYang, Jun-MoYou, Kwan-HeeKim, Chang-DeokLee, Jeung-Hoon
Keywords
Keratinocyte; PSIMAP; Protein-protein interaction network; SHC1; MAPK
Issue Date
2008-12
Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
Citation
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, v.377, no.2, pp.662 - 667
Abstract
Terminal differentiation of skin keratinocytes is a vertically directed multi-step process that is tightly controlled by the sequential expression of a variety of genes. We previously investigated the gene expression profile and found that many of differentiation-related genes expressed in a temporally regulated manner. In this study, we attempted to find the hub-molecules and their intracellular signaling networks during keratinocyte differentiation using in silico analysis of data obtained from previous studies. We used protein-protein interaction prediction software called PSIMAP, and drew a hypothetical signaling network. We chose one candidate hub-molecule SHC1 that were predicted to link EGFR and MAPK signal, and then evaluated the protein-protein interactions experimentally. As predicted, SHC1 bound to the MEK1 in an EGF-regulated manner. Furthermore, SHC1 bound to the MEK1 and p38 MAPK in a keratinocyte differentiation dependent manner. These results demonstrate that in silico protein-protein interaction prediction system can be used to efficiently and cost-effectively select the experimental candidates. (C) 2008 Elsevier Inc. All rights reserved
URI
Go to Link
DOI
10.1016/j.bbrc.2008.10.051
ISSN
0006-291X
Appears in Collections:
BME_Journal Papers
Files in This Item:
1-s2.0-S0006291X0802007X-main.pdf Download

find_unist can give you direct access to the published full text of this article. (UNISTARs only)

Show full item record

qrcode

  • mendeley

    citeulike

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

MENU