In vitro cytotoxicity screening of water-dispersible metal oxide nanoparticles in human cell lines
Cited 38 times inCited 39 times in
- In vitro cytotoxicity screening of water-dispersible metal oxide nanoparticles in human cell lines
- Choi, Jong Young; Lee, Su Hee; Na, Hyon Bin; An, Kwangjin; Hyeon, Taeghwan; Seo, Tae Seok
- Cytotoxicity; Magnetic resonance imaging contrast agents; Nanotoxicology; Water-dispersible Fe3O4 and MnO nanoparticle
- Issue Date
- BIOPROCESS AND BIOSYSTEMS ENGINEERING, v.33, no.1, pp.21 - 30
- In this study, we present in vitro cytotoxicity of iron oxide (Fe 3O4) and manganese oxide (MnO) using live/dead cell assay, lactate dehydrogenase assay, and reactive oxygen species detection with variation of the concentration of nanoparticles (5-500 μg/ml), incubation time (18-96 h), and different human cell lines (lung adenocarcinoma, breast cancer cells, and glioblastoma cells). The surface of nanoparticles is modified with polyethyleneglycol-derivatized phospholipid to enhance the biocompatibility, water-solubility, and stability under an aqueous media. While the cytotoxic effect was negligible for 18 h incubation even at highest concentration of 500 μg/ml, MnO nanoparticle represented higher level of toxicity than those of Fe3O4 and the commercial medical contrast reagent, Feridex after 2 and 4 day incubation time. However, the cytotoxicity of Fe3O4 is equivalent or better than Feridex based on the live/dead cell viability assay. The engineered MnO and Fe 3O4 exhibited excellent stability compared with Feridex for a prolonged incubation time. © 2009 Springer-Verlag.
- ; Go to Link
- Appears in Collections:
- ECHE_Journal Papers
- Files in This Item:
BBE 2010,33,21, In vitro cytotoxicity screening of water-dispersible metal oxide nanoparticles in human cell lines.pdf
can give you direct access to the published full text of this article. (UNISTARs only)
Show full item record
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.