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Ko, Myunggon
Cancer Epigenetics Lab.
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E2A/HEB and Id3 proteins control the sensitivity to glucocorticoidinduced apoptosis in thymocytes by regulating the SRG3 expression

Author(s)
Ko, Myung GonAhn, JeongeunLee, ChangjinChung, HeekyoungJeon, SunghoChung, Hee-YSeong, Rho H
Issued Date
2004-05
DOI
10.1074/jbc.M402145200
URI
https://scholarworks.unist.ac.kr/handle/201301/12657
Fulltext
http://www.jbc.org/content/279/21/21916.long
Citation
JOURNAL OF BIOLOGICAL CHEMISTRY, v.279, no.21, pp.21916 - 21923
Abstract
The E protein family transcription factors encoded by the E2A and HEB genes are known to play critical roles in the coordinate regulation of lymphocyte development. Previous studies have shown that T cell receptor (TCR) signals rapidly induce Id3, a dominant negative antagonist of E2A activity and allow thymocytes to survive selection events in the thymus. Here we show that SRG3 acts as a novel downstream target of E2A/HeLa E box-binding (HEB) complex and modulates glucocorticoid (GC) susceptibility in thymocytes in response to TCR signals. We have identified a putative E box element in the SRG3 promoter that is required for optimal promoter activity. The transcription factors E2A and HEB specifically associate with the E box element. Moreover, E2A-HEB heterodimers cooperated to activate SRG3 transcription, which was inhibited by the expression of Id proteins. TCR-mediated signals rapidly induced Id3 via MEK/ERK activation and thereby kept the E2A/HEB complex from binding to the E box element in the SRG3 promoter. Retroviral transduction of Id3 also repressed the SRG3 expression by inhibiting the E box binding activity of the E2A/HEB complex. Intriguingly, enforced Id3 expression conferred thymocyte resistance to GCs, which could be overcome by the overexpression of SRG3. Taken together, these results suggest that Id3 may enhance the viability of immature thymocytes by at least rendering them resistant to GCs through SRG3 down-regulation.
Publisher
AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
ISSN
0021-9258

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