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Kim, Jeong Beom
Molecular Biomedicine Lab
Research Interests
  • Patient-specific stem cells, induced pluripotent stem (iPS) cells, direct conversion, 3D bio-printing, Regenerative medicine, Patient-specific drug screening

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Erythroid differentiation of human induced pluripotent stem cells is independent of donor cell type of origin

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Title
Erythroid differentiation of human induced pluripotent stem cells is independent of donor cell type of origin
Author
Kim, Jeong BeomDorn, IsabelKlich, KatharinaArauzo-Bravo, Marcos J.Radstaak, MartinaSantourlidis, SimeonGhanjati, FouedRadke, Teja F.Psathaki, Olympia E.Hargus, GunnarKramer, JanEinhaus, MartinKogler, G esineWernet, PeterScholer, Hans R.Schlenke, PeterZaehres, Holm
Issue Date
2015-01
Publisher
FERRATA STORTI FOUNDATION
Citation
HAEMATOLOGICA-THE HEMATOLOGY JOURNAL, v.100, no.1, pp.32 - 41
Abstract
Epigenetic memory in induced pluripotent stem cells, which is related to the somatic cell type of origin of the stem cells, might lead to variations in the differentiation capacities of the pluripotent stem cells. In this context, induced pluripotent stem cells from human CD34(+) hematopoietic stem cells might be more suitable for hematopoietic differentiation than the commonly used fibroblast-derived induced pluripotent stem cells. To investigate the influence of an epigenetic memory on the ex vivo expansion of induced pluripotent stem cells into erythroid cells, we compared induced pluripotent stem cells from human neural stem cells and human cord blood-derived CD34(+) hematopoietic stem cells and evaluated their potential for differentiation into hematopoietic progenitor and mature red blood cells. Although genome-wide DNA methylation profiling at all promoter regions demonstrates that the epigenetic memory of induced pluripotent stem cells is influenced by the somatic cell type of origin of the stem cells, we found a similar hematopoietic induction potential and erythroid differentiation pattern of induced pluripotent stem cells of different somatic cell origin. All human induced pluripotent stem cell lines showed terminal maturation into normoblasts and enucleated reticulocytes, producing predominantly fetal hemoglobin. Differences were only observed in the growth rate of erythroid cells, which was slightly higher in the induced pluripotent stem cells derived from CD34(+) hematopoietic stem cells. More detailed methylation analysis of the hematopoietic and erythroid promoters identified similar CpG methylation levels in the induced pluripotent stem cell lines derived from CD34(+) cells and those derived from neural stem cells, which confirms their comparable erythroid differentiation potential.
URI
https://scholarworks.unist.ac.kr/handle/201301/10809
URL
http://www.haematologica.org/content/100/1/32.full-text.pdf+html
DOI
10.3324/haematol.2014.108068
ISSN
0390-6078
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BME_Journal Papers
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