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Kim, Jeong Beom
Molecular Biomedicine Lab
Research Interests
  • Patient-specific stem cells, induced pluripotent stem (iPS) cells, direct conversion, 3D bio-printing, Regenerative medicine, Patient-specific drug screening

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Erythroid differentiation of human induced pluripotent stem cells is independent of donor cell type of origin

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dc.contributor.author Kim, Jeong Beom ko
dc.contributor.author Dorn, Isabel ko
dc.contributor.author Klich, Katharina ko
dc.contributor.author Arauzo-Bravo, Marcos J. ko
dc.contributor.author Radstaak, Martina ko
dc.contributor.author Santourlidis, Simeon ko
dc.contributor.author Ghanjati, Foued ko
dc.contributor.author Radke, Teja F. ko
dc.contributor.author Psathaki, Olympia E. ko
dc.contributor.author Hargus, Gunnar ko
dc.contributor.author Kramer, Jan ko
dc.contributor.author Einhaus, Martin ko
dc.contributor.author Kogler, G esine ko
dc.contributor.author Wernet, Peter ko
dc.contributor.author Scholer, Hans R. ko
dc.contributor.author Schlenke, Peter ko
dc.contributor.author Zaehres, Holm ko
dc.date.available 2015-03-09T00:20:36Z -
dc.date.created 2015-02-05 ko
dc.date.issued 2015-01 ko
dc.identifier.citation HAEMATOLOGICA-THE HEMATOLOGY JOURNAL, v.100, no.1, pp.32 - 41 ko
dc.identifier.issn 0390-6078 ko
dc.identifier.uri https://scholarworks.unist.ac.kr/handle/201301/10809 -
dc.description.abstract Epigenetic memory in induced pluripotent stem cells, which is related to the somatic cell type of origin of the stem cells, might lead to variations in the differentiation capacities of the pluripotent stem cells. In this context, induced pluripotent stem cells from human CD34(+) hematopoietic stem cells might be more suitable for hematopoietic differentiation than the commonly used fibroblast-derived induced pluripotent stem cells. To investigate the influence of an epigenetic memory on the ex vivo expansion of induced pluripotent stem cells into erythroid cells, we compared induced pluripotent stem cells from human neural stem cells and human cord blood-derived CD34(+) hematopoietic stem cells and evaluated their potential for differentiation into hematopoietic progenitor and mature red blood cells. Although genome-wide DNA methylation profiling at all promoter regions demonstrates that the epigenetic memory of induced pluripotent stem cells is influenced by the somatic cell type of origin of the stem cells, we found a similar hematopoietic induction potential and erythroid differentiation pattern of induced pluripotent stem cells of different somatic cell origin. All human induced pluripotent stem cell lines showed terminal maturation into normoblasts and enucleated reticulocytes, producing predominantly fetal hemoglobin. Differences were only observed in the growth rate of erythroid cells, which was slightly higher in the induced pluripotent stem cells derived from CD34(+) hematopoietic stem cells. More detailed methylation analysis of the hematopoietic and erythroid promoters identified similar CpG methylation levels in the induced pluripotent stem cell lines derived from CD34(+) cells and those derived from neural stem cells, which confirms their comparable erythroid differentiation potential. ko
dc.description.statementofresponsibility close -
dc.language 영어 ko
dc.publisher FERRATA STORTI FOUNDATION ko
dc.title Erythroid differentiation of human induced pluripotent stem cells is independent of donor cell type of origin ko
dc.type ARTICLE ko
dc.identifier.scopusid 2-s2.0-84920111966 ko
dc.identifier.wosid 000351278500020 ko
dc.type.rims ART ko
dc.description.wostc 1 *
dc.description.scopustc 0 *
dc.date.tcdate 2015-12-28 *
dc.date.scptcdate 2015-11-04 *
dc.identifier.doi 10.3324/haematol.2014.108068 ko
dc.identifier.url http://www.haematologica.org/content/100/1/32.full-text.pdf+html ko
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