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Bhak, Jong
KOrean GenomIcs Center(KOGIC)
Research Interests
  • Geromics, genomics, bioinformatics, protein Engineering, OMICS

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Identification and Characterization of MicroRNAs in Normal Equine Tissues by Next Generation Sequencing

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Title
Identification and Characterization of MicroRNAs in Normal Equine Tissues by Next Generation Sequencing
Author
Kim, Myung-ChulLee, Seung-WooRyu, Doug-YoungCui, Feng-JiBhak, JongKim, Yongbaek
Keywords
BIOINFORMATICS ANALYSIS; ADIPOSE-TISSUE; DOMESTIC HORSE; MAMMARY-GLAND; EXPRESSION; RNA; PRINCIPLES; DISORDERS; MIR-17-92; CARTILAGE
Issue Date
2014-04
Publisher
PUBLIC LIBRARY SCIENCE
Citation
PLOS ONE, v.9, no.4, pp.1 - 10
Abstract
The role of microRNAs (miRNAs) as a post-transcriptional gene regulator has been elucidated in a broad range of organisms including domestic animals. Characterization of miRNAs in normal tissues is an important step to investigate the functions of miRNAs in various physiological and pathological conditions. Using Illumina Next Generation Sequencing (NGS) technology, we identified a total of 292 known and 329 novel miRNAs in normal horse tissues including skeletal muscle, colon and liver. Distinct sets of miRNAs were differentially expressed in a tissue-specific manner. The miRNA genes were distributed across all the chromosomes except chromosomes 29 and 31 in the horse reference genome. In some chromosomes, multiple miRNAs were clustered and considered to be polycistronic transcript. A base composition analysis showed that equine miRNAs had a higher frequency of A+U than G+C. Furthermore, U tended to be more frequent at the 59 end of miRNA sequences. This is the first experimental study that identifies and characterizes the global miRNA expression profile in normal horse tissues. The present study enriches the horse miRNA database and provides useful information for further research dissecting biological functions of miRNAs in horse.
URI
https://scholarworks.unist.ac.kr/handle/201301/10164
DOI
10.1371/journal.pone.0093662
ISSN
1932-6203
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BME_Journal Papers
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