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Bhak, Jong
KOrean GenomIcs Center(KOGIC)
Research Interests
  • Geromics, genomics, bioinformatics, protein Engineering, OMICS


Identification and Characterization of MicroRNAs in Normal Equine Tissues by Next Generation Sequencing

DC Field Value Language Kim, Myung-Chul ko Lee, Seung-Woo ko Ryu, Doug-Young ko Cui, Feng-Ji ko Bhak, Jong ko Kim, Yongbaek ko 2015-01-19T00:05:09Z - 2014-10-15 ko 2014-04 -
dc.identifier.citation PLOS ONE, v.9, no.4, pp.1 - 10 ko
dc.identifier.issn 1932-6203 ko
dc.identifier.uri -
dc.description.abstract The role of microRNAs (miRNAs) as a post-transcriptional gene regulator has been elucidated in a broad range of organisms including domestic animals. Characterization of miRNAs in normal tissues is an important step to investigate the functions of miRNAs in various physiological and pathological conditions. Using Illumina Next Generation Sequencing (NGS) technology, we identified a total of 292 known and 329 novel miRNAs in normal horse tissues including skeletal muscle, colon and liver. Distinct sets of miRNAs were differentially expressed in a tissue-specific manner. The miRNA genes were distributed across all the chromosomes except chromosomes 29 and 31 in the horse reference genome. In some chromosomes, multiple miRNAs were clustered and considered to be polycistronic transcript. A base composition analysis showed that equine miRNAs had a higher frequency of A+U than G+C. Furthermore, U tended to be more frequent at the 59 end of miRNA sequences. This is the first experimental study that identifies and characterizes the global miRNA expression profile in normal horse tissues. The present study enriches the horse miRNA database and provides useful information for further research dissecting biological functions of miRNAs in horse. ko
dc.description.statementofresponsibility open -
dc.language ENG ko
dc.subject ADIPOSE-TISSUE ko
dc.subject DOMESTIC HORSE ko
dc.subject MAMMARY-GLAND ko
dc.subject EXPRESSION ko
dc.subject RNA ko
dc.subject PRINCIPLES ko
dc.subject DISORDERS ko
dc.subject MIR-17-92 ko
dc.subject CARTILAGE ko
dc.title Identification and Characterization of MicroRNAs in Normal Equine Tissues by Next Generation Sequencing ko
dc.type ARTICLE ko
dc.identifier.scopusid 2-s2.0-84898927278 ko
dc.identifier.wosid 000334103000099 ko
dc.type.rims ART ko
dc.description.wostc 2 *
dc.description.scopustc 2 * 2015-12-28 * 2015-11-04 *
dc.identifier.doi 10.1371/journal.pone.0093662 ko
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