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Suh, Pann-Ghill
BioSignal Network Lab (BSN)
Research Interests
  • Signal transduction, cancer, metabolism, phospholipase C

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Ligand profiling and identification technology for searching bioactive ligands

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Title
Ligand profiling and identification technology for searching bioactive ligands
Author
Baek, MCKim, SJYea, KKim, YLee, BDKim, JLee, HJKang, MHChoi, SKKim, JILee, TGSuh, Pann-GhillRyu, SH
Issue Date
2006-03
Publisher
WILEY-BLACKWELL
Citation
PROTEOMICS, v.6, no.6, pp.1741 - 1749
Abstract
We introduce a new methodology named ligand profiling and identification for effective discovery of bioactive ligands such as peptide hormones. This technology was developed from a new concept of parallel column chromatography and active fraction profiling by nano-LC MS. Traditional methods use sequential column chromatography, and thus are inevitably limited by the low abundance of the peptide of interest and by a low yield due to the many column steps. Using this new technology, insulin was successfully identified and diarginylinsulin, a minor intermediate form of insulin, was unexpectedly also identified simultaneously from 100 mg of porcine pancreatic tissue. This integrative technology could be used to search for various low-abundance peptides (or bioactive molecules) rapidly and simultaneously, by applying this to the later stages of traditional sequential purification.
URI
https://scholarworks.unist.ac.kr/handle/201301/10126
URL
http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=33645472063
DOI
10.1002/pmic.200500511
ISSN
1615-9853
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BIO_Journal Papers
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