Ligand profiling and identification technology for searching bioactive ligands
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- Ligand profiling and identification technology for searching bioactive ligands
- Baek, MC; Kim, SJ; Yea, K; Kim, Y; Lee, BD; Kim, J; Lee, HJ; Kang, MH; Choi, SK; Kim, JI; Lee, TG; Suh, Pann-Ghill; Ryu, SH
- Issue Date
- PROTEOMICS, v.6, no.6, pp.1741 - 1749
- We introduce a new methodology named ligand profiling and identification for effective discovery of bioactive ligands such as peptide hormones. This technology was developed from a new concept of parallel column chromatography and active fraction profiling by nano-LC MS. Traditional methods use sequential column chromatography, and thus are inevitably limited by the low abundance of the peptide of interest and by a low yield due to the many column steps. Using this new technology, insulin was successfully identified and diarginylinsulin, a minor intermediate form of insulin, was unexpectedly also identified simultaneously from 100 mg of porcine pancreatic tissue. This integrative technology could be used to search for various low-abundance peptides (or bioactive molecules) rapidly and simultaneously, by applying this to the later stages of traditional sequential purification.
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