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DC Field | Value | Language |
---|---|---|
dc.citation.endPage | 1749 | - |
dc.citation.number | 6 | - |
dc.citation.startPage | 1741 | - |
dc.citation.title | PROTEOMICS | - |
dc.citation.volume | 6 | - |
dc.contributor.author | Baek, MC | - |
dc.contributor.author | Kim, SJ | - |
dc.contributor.author | Yea, K | - |
dc.contributor.author | Kim, Y | - |
dc.contributor.author | Lee, BD | - |
dc.contributor.author | Kim, J | - |
dc.contributor.author | Lee, HJ | - |
dc.contributor.author | Kang, MH | - |
dc.contributor.author | Choi, SK | - |
dc.contributor.author | Kim, JI | - |
dc.contributor.author | Lee, TG | - |
dc.contributor.author | Suh, Pann-Ghill | - |
dc.contributor.author | Ryu, SH | - |
dc.date.accessioned | 2023-12-22T10:07:37Z | - |
dc.date.available | 2023-12-22T10:07:37Z | - |
dc.date.created | 2015-01-14 | - |
dc.date.issued | 2006-03 | - |
dc.description.abstract | We introduce a new methodology named ligand profiling and identification for effective discovery of bioactive ligands such as peptide hormones. This technology was developed from a new concept of parallel column chromatography and active fraction profiling by nano-LC MS. Traditional methods use sequential column chromatography, and thus are inevitably limited by the low abundance of the peptide of interest and by a low yield due to the many column steps. Using this new technology, insulin was successfully identified and diarginylinsulin, a minor intermediate form of insulin, was unexpectedly also identified simultaneously from 100 mg of porcine pancreatic tissue. This integrative technology could be used to search for various low-abundance peptides (or bioactive molecules) rapidly and simultaneously, by applying this to the later stages of traditional sequential purification. | - |
dc.identifier.bibliographicCitation | PROTEOMICS, v.6, no.6, pp.1741 - 1749 | - |
dc.identifier.doi | 10.1002/pmic.200500511 | - |
dc.identifier.issn | 1615-9853 | - |
dc.identifier.scopusid | 2-s2.0-33645472063 | - |
dc.identifier.uri | https://scholarworks.unist.ac.kr/handle/201301/10126 | - |
dc.identifier.url | http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=33645472063 | - |
dc.identifier.wosid | 000236420600007 | - |
dc.language | 영어 | - |
dc.publisher | WILEY-BLACKWELL | - |
dc.title | Ligand profiling and identification technology for searching bioactive ligands | - |
dc.type | Article | - |
dc.description.journalRegisteredClass | scopus | - |
dc.subject.keywordAuthor | identification | - |
dc.subject.keywordAuthor | ligand | - |
dc.subject.keywordAuthor | peptidome | - |
dc.subject.keywordAuthor | profiling | - |
dc.subject.keywordPlus | HUMAN INSULIN-RECEPTOR | - |
dc.subject.keywordPlus | PROTEIN-COUPLED RECEPTORS | - |
dc.subject.keywordPlus | HUMAN-GENOME-PROJECT | - |
dc.subject.keywordPlus | MASS-SPECTROMETRY | - |
dc.subject.keywordPlus | CLEAVAGE SITES | - |
dc.subject.keywordPlus | KINASE | - |
dc.subject.keywordPlus | DIARGINYLINSULIN | - |
dc.subject.keywordPlus | PROINSULIN | - |
dc.subject.keywordPlus | PROTEOMICS | - |
dc.subject.keywordPlus | PEPTIDE | - |
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