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Suh, Pann-Ghill
BioSignal Network Lab (BSN)
Research Interests
  • Signal transduction, cancer, metabolism, phospholipase C

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The promoter activity of the phospholipase C-gamma 2 gene is regulated by a cell-type-specific control element

Cited 4 times inthomson ciCited 4 times inthomson ci
Title
The promoter activity of the phospholipase C-gamma 2 gene is regulated by a cell-type-specific control element
Author
Lee, SJBahk, YYYun, DHLee, HJLee, YHRyu, SHSuh, Pann-Ghill
Keywords
MURINE LYMPHOCYTES-B; TYROSINE KINASE; ELEVATED CONTENT; C ISOZYMES; TRANSCRIPTION; EXPRESSION; PROTEIN; DNA; DIFFERENTIATION; PHOSPHORYLATION
Issue Date
199704
Publisher
MARY ANN LIEBERT INC
Citation
DNA AND CELL BIOLOGY, v.16, no.4, pp.485 - 492
Abstract
We have cloned and characterized a genomic DNA spanning the 5'-flanking region, the first and second exons, and the first intron of the human PLC-gamma 2 gene. The proximal upstream region is highly CC-rich and lacks a TATA box, whereas the distal region contains several AT-rich tracts. Multiple transcription initiation sites were identified by primer extension analysis, Based on the transient transfection assays, the major transcriptional activation element was identified between -183 and +43 (G2SE) and a transcriptional repressive element was found between -303 and -184 (G2RE). The expression of PLC-gamma 2 in various cell lines was examined using monoclonal anti-PLC-gamma 2 antibody, PLC-gamma 2 was highly expressed in B-cell lines such as Daudi, SP2, and Ramos cells, whereas it existed at very low levels in Jurkat, 3T3-L1, NBL-7, and C6Bu-1 cells. Moderate levels of PLC-gamma 2 were also detected in C2C12, P19, U937, HL60, A431, and PC12 cells. The 4-kb genomic fragment upstream of -1,654 was able to activate transcription from the PLC-gamma 2 promoter in Daudi and C2C12 cells, but not in Jurkat cells, which is consistent with the PLC-gamma 2 protein expression levels in those cell lines. These results suggest that the cell-type-specific expression of PLC-gamma 2 might be attributed to the transcriptional regulation by the upstream cis-element.
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DOI
http://dx.doi.org/10.1089/dna.1997.16.485
ISSN
1044-5498
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