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DC Field | Value | Language |
---|---|---|
dc.citation.endPage | 492 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 485 | - |
dc.citation.title | DNA AND CELL BIOLOGY | - |
dc.citation.volume | 16 | - |
dc.contributor.author | Lee, SJ | - |
dc.contributor.author | Bahk, YY | - |
dc.contributor.author | Yun, DH | - |
dc.contributor.author | Lee, HJ | - |
dc.contributor.author | Lee, YH | - |
dc.contributor.author | Ryu, SH | - |
dc.contributor.author | Suh, Pann-Ghill | - |
dc.date.accessioned | 2023-12-22T12:37:39Z | - |
dc.date.available | 2023-12-22T12:37:39Z | - |
dc.date.created | 2014-09-03 | - |
dc.date.issued | 1997-04 | - |
dc.description.abstract | We have cloned and characterized a genomic DNA spanning the 5'-flanking region, the first and second exons, and the first intron of the human PLC-gamma 2 gene. The proximal upstream region is highly CC-rich and lacks a TATA box, whereas the distal region contains several AT-rich tracts. Multiple transcription initiation sites were identified by primer extension analysis, Based on the transient transfection assays, the major transcriptional activation element was identified between -183 and +43 (G2SE) and a transcriptional repressive element was found between -303 and -184 (G2RE). The expression of PLC-gamma 2 in various cell lines was examined using monoclonal anti-PLC-gamma 2 antibody, PLC-gamma 2 was highly expressed in B-cell lines such as Daudi, SP2, and Ramos cells, whereas it existed at very low levels in Jurkat, 3T3-L1, NBL-7, and C6Bu-1 cells. Moderate levels of PLC-gamma 2 were also detected in C2C12, P19, U937, HL60, A431, and PC12 cells. The 4-kb genomic fragment upstream of -1,654 was able to activate transcription from the PLC-gamma 2 promoter in Daudi and C2C12 cells, but not in Jurkat cells, which is consistent with the PLC-gamma 2 protein expression levels in those cell lines. These results suggest that the cell-type-specific expression of PLC-gamma 2 might be attributed to the transcriptional regulation by the upstream cis-element. | - |
dc.identifier.bibliographicCitation | DNA AND CELL BIOLOGY, v.16, no.4, pp.485 - 492 | - |
dc.identifier.doi | 10.1089/dna.1997.16.485 | - |
dc.identifier.issn | 1044-5498 | - |
dc.identifier.scopusid | 2-s2.0-0030965863 | - |
dc.identifier.uri | https://scholarworks.unist.ac.kr/handle/201301/5621 | - |
dc.identifier.url | http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=0030965863 | - |
dc.identifier.wosid | A1997WY50500012 | - |
dc.language | 영어 | - |
dc.publisher | MARY ANN LIEBERT INC | - |
dc.title | The promoter activity of the phospholipase C-gamma 2 gene is regulated by a cell-type-specific control element | - |
dc.type | Article | - |
dc.description.journalRegisteredClass | scopus | - |
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